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JOURNAL ARTICLES
A Xenopus nodal-related gene that acts in synergy with noggin to induce complete secondary axis and notochord formation
K.D. Lustig, K. Kroll, E. Sun, R. Ramos, H. Elmendorf, M.W. Kirschner
Development 1996 122: 3275-3282;

Summary

Using a paracrine assay to screen for signaling proteins that could respecify ectodermal tissue, we isolated a Xenopus gene related to the mouse gene nodal, a member of the TGFbeta superfamily. The gene is expressed in three regions in the early Xenopus embryo: first in the gastrula organizer, then in two stripes of cells flanking the posterior notochord in late neurulae, and finally in lateral plate mesoderm restricted to the left side of tailbud-stage embryos. Ectopic expression of the gene induces muscle formation in ectodermal explants and partial secondary axes in whole embryos. Together with noggin, another secreted protein also present in the organizer, it induces notochord formation in ectodermal explants and complete secondary axes in whole embryos. These results suggest that the nodal-related gene may act together with noggin to induce axial pattern during gastrulation and also may play a role in left-right asymmetry generation in the post-gastrula embryo.

Reference

    1. Bolce M. E.,
    2. Hemmati-Brivanlou A.,
    3. Kushner P. D.,
    4. Harland R. M.
    (1992) Ventral ectoderm of Xenopus forms neural tissue, including hindbrain, in response to activin. Development 115, 681–688
    OpenUrlAbstract
    1. Conlon F. L.,
    2. Lyons K. M.,
    3. Takaesu N.,
    4. Barth K. S.,
    5. Kispert A.,
    6. Hermann B.,
    7. Robertson E. J.
    (1994) A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse. Development 120, 1919–1928
    OpenUrlAbstract
    1. Ecochard V.,
    2. Cayrol C.,
    3. Foulquier F.,
    4. Zaraisky A.,
    5. Duprat A. M.
    (1995) A Novel TGF--like gene, fugacin, specifically expressed in the Spemann organizer of Xenopus. Dev. Biol 172, 699–703
    OpenUrlCrossRefPubMed
    1. Harland R. M.
    (1991) In situ hybridization: an improved whole-mount method for Xenopus embryos. Meth. Cell Biol 36, 685–695
    OpenUrlCrossRefPubMedWeb of Science
    1. Hemmati-Brivanlou A.,
    2. Kelly O. G.,
    3. Melton D. A.
    (1994) Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activity. Cell 77, 283–295
    OpenUrlCrossRefPubMedWeb of Science
    1. Hemmati-Brivanlou A.,
    2. Melton D. A.
    (1994) Inhibition of activin receptor signaling promotes neuralization in Xenopus. Cell 77, 273–282
    OpenUrlCrossRefPubMedWeb of Science
    1. Jones C. M.,
    2. Kuehn M. R.,
    3. Hogan B. L. M.,
    4. Smith J. C.,
    5. Wright C. V. E.
    (1995) Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation. Development 121, 3651–3662
    OpenUrlAbstract
    1. Kao K. R.,
    2. Elinson R. P.
    (1988) The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos. Dev. Biol 127, 64–77
    OpenUrlCrossRefPubMedWeb of Science
    1. Kingsley D. M.
    (1994) The TGF-superfamily: new members, new receptors, and new genetic tests of function in different organisms. Genes Dev 8, 133–146
    OpenUrlFREE Full Text
    1. Kintner C. R.,
    2. Brockes J. P.
    (1984) Monoclonal antibodies identify blastemal cells derived from dedifferentiating muscle in newt limb regeneration. Nature 308, 67–69
    OpenUrlCrossRefPubMed
    1. Lemaire P.,
    2. Garrett N.,
    3. Gurdon J. B.
    (1995) Expression cloning of Siamois, a Xenopus homeobox gene expressed in dorsal-vegetal cells of blastulae and able to induce a complete secondary axis. Cell 81, 85–94
    OpenUrlCrossRefPubMedWeb of Science
    1. Levin M.,
    2. Johnson R. L.,
    3. Stern C. D.,
    4. Kuehn M.,
    5. Tabin C.
    (1995) A molecular pathway determining left-right asymmetry in chick embryogenesis. Cell 82, 803–814
    OpenUrlCrossRefPubMedWeb of Science
    1. Lustig K. D.,
    2. Kirschner M. W.
    (1995) Use of an oocyte expression assay to reconstitute inductive signaling. Proc. Nat. Acad. Sci. USA 92, 6234–6238
    OpenUrlAbstract/FREE Full Text
    1. McMahon A. P.,
    2. Moon R. T.
    (1989) Ectopic expression of the proto-oncogene int-1 in Xenopus embryos leads to duplication of the embryonic axis. Cell 58, 1075–1084
    OpenUrlCrossRefPubMedWeb of Science
    1. Nakamura T.,
    2. Takio K.,
    3. Eto Y.,
    4. Shibai H.,
    5. Titani K.,
    6. Sugino H.
    (1990) Activin-binding protein from rat ovary is follistatin. Science 247, 836–838
    OpenUrlAbstract/FREE Full Text
    1. Parr B. A.,
    2. McMahon A. P.
    (1994) Wnt genes and vertebrate development. Curr. Opin. Genet. Dev 4, 523–528
    OpenUrlCrossRefPubMed
    1. Rupp R. A.,
    2. Weintraub H.
    (1991) Ubiquitous MyoD transcription at the midblastula transition precedes induction-dependent MyoD expression in presumptive mesoderm of X. laevis. Cell 65, 927–937
    OpenUrlCrossRefPubMedWeb of Science
    1. Sasai Y.,
    2. Lu B.,
    3. Steinbeisser H.,
    4. Geissert D.,
    5. Gont L. K.,
    6. De R. E.
    (1994) Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes. Cell 79, 779–790
    OpenUrlCrossRefPubMedWeb of Science
    1. Smith W. C.,
    2. Harland R. M.
    (1992) Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos. Cell 70, 829–840
    OpenUrlCrossRefPubMedWeb of Science
    1. Smith W. C.,
    2. McKendry R.,
    3. Ribisi S. J.,
    4. Harland R. M.
    (1995) A nodal-related gene defines a physical and functional domain within the Spemann organizer. Cell 82, 37–46
    OpenUrlCrossRefPubMedWeb of Science
    1. Wilson P. A.,
    2. Melton D. A.
    (1994) Mesodermal patterning by an inducer gradient depends on secondary cell-cell communication. Curr. Biol 4, 676–686
    OpenUrlCrossRefPubMedWeb of Science
    1. Zhou X.,
    2. Sasaki H.,
    3. Lowe L.,
    4. Hogan B. L.,
    5. Kuehn M. R.
    (1993) Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation. Nature 361, 543–547
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JOURNAL ARTICLES
A Xenopus nodal-related gene that acts in synergy with noggin to induce complete secondary axis and notochord formation
K.D. Lustig, K. Kroll, E. Sun, R. Ramos, H. Elmendorf, M.W. Kirschner
Development 1996 122: 3275-3282;
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