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JOURNAL ARTICLES
Role of Bicaudal-D in patterning the Drosophila egg chamber in mid-oogenesis
A. Swan, B. Suter
Development 1996 122: 3577-3586;

Summary

The Bicaudal-D (Bic-D) gene is required early in Drosophila oogenesis for the differentiation of an oocyte from one of a cluster of 16 interconnected germarial cells. To analyze the role of Bic-D later in oogenesis, we have constructed Drosophila lines in which Bic-D expression is under the control of the hsp70 promoter. In these flies, Bic-D activity can be induced early in oogenesis, allowing an oocyte to be made. Then, by shifting females to non-inducing conditions, Bic-D levels are depleted for the remainder of oogenesis. Using this system, we find that Bic-D is indeed required in the later stages of oogenesis. In ovaries from mutant females, oocyte growth is reduced, apparently due to defects in nurse-cell-to-oocyte transport. Smaller oocyte size results in the misalignment of follicle cells and the underlying germ line, leading to ventralization of dorsal follicle cells and to defects in centripetal cell migration. In addition, we show that Bic-D is required for the localization of specific mRNAs at both the anterior and posterior of the oocyte.

Reference

    1. Bohrmann J.,
    2. Biber K.
    (1994) Cytoskeleton-dependent transport of cytoplasmic particles in previtellogenic to mid-vitellogenic ovarian follicles of Drosophila: Time-lapse analysis using video-enhanced contrast microscopy. J. Cell Sci 107, 849–858
    OpenUrlAbstract/FREE Full Text
    1. Brand A. H.,
    2. Perrimon N.
    (1994) Raf acts downstream of the EGF receptor to determine dorsoventral polarity during Drosophila oogenesis. Genes Dev 8, 629–639
    OpenUrlAbstract/FREE Full Text
    1. Cheung H.-K.,
    2. Serano T. L.,
    3. Cohen R. S.
    (1992) Evidence for a highly selective RNA transport system and its role in establishing the dorsoventral axis of the Drosophila egg. Development 114, 653–661
    OpenUrlAbstract
    1. Clark I.,
    2. Giniger E.,
    3. Ruohola-Baker H.,
    4. Jan L. Y.,
    5. Jan Y. N.
    (1994) Transient posterior localization of a kinesin fusion protein reflects anteroposterior polarity of the Drosophila oocyte. Current Biol 4, 289–300
    OpenUrlCrossRefPubMedWeb of Science
    1. Cooley L.,
    2. Theurkauf W.E.
    (1994) Cytoskeletal functions during Drosophila oogenesis. Science 266, 590–596
    OpenUrlAbstract/FREE Full Text
    1. Ephrussi A.,
    2. Dickinson L. K.,
    3. Lehmann R.
    (1991) osk organizes the germ plasm and directs localization of the posterior determinant nanos. Cell 66, 37–50
    OpenUrlCrossRefPubMedWeb of Science
    1. González-Reyes A.,
    2. St. Johnston R. D.
    (1994) Role of oocyte position in establishment of anterior-posterior polarity in Drosophila. Science 266, 639–642
    OpenUrlAbstract/FREE Full Text
    1. Kellum R.,
    2. Schedl P.
    (1991) A positon-effect assay for boundaries of higher order chromosomal domains. Cell 64, 941–950
    OpenUrlCrossRefPubMedWeb of Science
    1. King R. C.,
    2. Burnett R. G.
    (1959) An autoradiographic study of uptake of tritiated glycine, thymidine, and uridine by fruit fly ovaries. Science 129, 1674–1675
    OpenUrlAbstract/FREE Full Text
    1. Kim-Ha J.,
    2. Smith J. L.,
    3. Macdonald P. M.
    (1991) osk mRNA is localized to the posterior pole of the Drosophila oocyte. Cell 66, 23–35
    OpenUrlCrossRefPubMedWeb of Science
    1. Koch E. A.,
    2. Spitzer R. H.
    (1983) Multiple effects of colchicine on oogenesis in Drosophila: Induced sterility and switch of potential oocyte to nurse-cell developmental pathway. Cell Tissue Res 228, 21–32
    OpenUrlPubMedWeb of Science
    1. Lane M. E.,
    2. Kalderon D.
    (1994) RNA localization along the anteroposterior axis of the Drosophila oocyte requires PKA-mediated signal transduction to direct normal microtubule organization. Genes Dev 8, 2986–2995
    OpenUrlAbstract/FREE Full Text
    1. Lantz V.,
    2. Ambrosio L.,
    3. Schedl P.
    (1992) The Drosophilaorb gene is predicted to encode sex-specific germline RNA-binding proteins and has localized transcripts in ovaries and early embryos. Development 115, 75–88
    OpenUrlAbstract
    1. Lis J. T.,
    2. Simon J. A.,
    3. Sutton C. A.
    (1983) New heat shock puffs and-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene. Cell 35, 403–410
    OpenUrlCrossRefPubMedWeb of Science
    1. Mohler J.,
    2. Wieschaus E. F.
    (1986) Dominant maternal-effect mutations of Drosophilamelanogaster causing the production of double-abdomen embryos. Genetics 112, 803–822
    OpenUrlAbstract/FREE Full Text
    1. Neuman-Silberberg F. S.,
    2. Schupbach T.
    (1993) The Drosophila dorsoventral patterning gene gurken produces a dorsally localized RNA and encodes a TGFa-like protein. Cell 75, 165–174
    OpenUrlCrossRefPubMedWeb of Science
    1. Pierre P.,
    2. Scheel J.,
    3. Rickard J. E.,
    4. Kreis T. E.
    (1992) CLIP-170 links endocytic vesicles to microtubules. Cell 70, 887–900
    OpenUrlCrossRefPubMedWeb of Science
    1. Pokrywka N. J.,
    2. Stephenson E. C.
    (1991) Microtubules mediate the localization of bicoid RNA during Drosophila oogenesis. Development 113, 55–66
    OpenUrlAbstract
    1. Pokrywka N. J.,
    2. Stephenson E. C.
    (1995) Microtubules are a general component of mRNA localization systems in Drosophila oocytes. Dev. Biol 167, 363–370
    OpenUrlCrossRefPubMedWeb of Science
    1. Ran B.,
    2. Bopp R.,
    3. Suter B.
    (1994) Null alleles reveal novel requirements for Bic-D during Drosophila oogenesis and zygotic development. Development 120, 1233–1242
    OpenUrlAbstract
    1. Roth S.,
    2. Schupbach T.
    (1994) The relationship between ovarian and embryonic dorsoventral patterning in Drosophila. Development 120, 2245–2257
    OpenUrlAbstract
    1. Schroer T. A.,
    2. Sheetz M. P.
    (1991) Two activators of microtubule-based vesicle transport. J. Cell Biol 115, 1390–1318
    OpenUrl
    1. Schupbach T.
    (1987) Germ line and soma cooperate during oogenesis to establish the dorsoventral pattern of egg shell and embryo in Drosophila melanogaster. Cell 49, 699–707
    OpenUrlCrossRefPubMedWeb of Science
    1. Schupbach T.,
    2. Wieschaus E.
    (1991) Female sterile mutations on the second chromosome of Drosophilamelanogaster: II Mutations blocking oogenesis or altering egg morphology. Genetics 129, 1119–1136
    OpenUrlAbstract/FREE Full Text
    1. Schupbach T.,
    2. Roth S.
    (1994) Dorsoventral patterning in Drosophila oogenesis. Curr. Opin. Genet. Dev 4, 502–507
    OpenUrlCrossRefPubMed
    1. Suter B.,
    2. Romberg L. M.,
    3. Steward R.
    (1989) Bicaudal-D, a Drosophila gene involved in developmental asymmetry: localized transcript accumulation in ovaries and sequence similarity to myosin heavy chain tail domains. Genes Dev 3, 1957–1968
    OpenUrlAbstract/FREE Full Text
    1. Suter B.,
    2. Steward R.
    (1991) Requirement for phosphorylation andlocalization of the Bicaudal-D protein in Drosophila oocyte differentiation. Cell 67, 917–926
    OpenUrlCrossRefPubMedWeb of Science
    1. Theurkauf W. E.,
    2. Smiley S.,
    3. Wong M. L.,
    4. Alberts B. M.
    (1992) Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport. Development 115, 923–936
    OpenUrlAbstract
    1. Theurkauf W. E.,
    2. Alberts B. M.,
    3. Jan Y. N.,
    4. Jongens T. A.
    (1993) A central role for microtubules in the differentiation of Drosophila oocytes. Development 118, 1169–1180
    OpenUrlAbstract/FREE Full Text
    1. Wharton R. P.,
    2. Struhl G.
    (1989) Structure of the Drosophila Bicaudal-D protein and its role in localizing the posterior determinant nanos. Cell 59, 881–892
    OpenUrlCrossRefPubMedWeb of Science
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JOURNAL ARTICLES
Role of Bicaudal-D in patterning the Drosophila egg chamber in mid-oogenesis
A. Swan, B. Suter
Development 1996 122: 3577-3586;
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