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JOURNAL ARTICLES
The Drosophila stonewall gene encodes a putative transcription factor essential for germ cell development
K.A. Clark, D.M. McKearin
Development 1996 122: 937-950;

Summary

The differentiation of Drosophila germ cells is a useful model for studying mechanisms of cell specification. We report the identification of a gene, stonewall, that is required for germ cell development. Mutations in stonewall block proper oocyte differentiation and frequently cause the presumptive oocyte to develop as a nurse cell. Eventually, germ cells degenerate apoptotically. Stonewall is a germ cell nuclear protein; Stonewall has a DNA binding domain that shows similarities to the Myb and Adf-1 transcription factors and has other features that suggest that it is a transcription activating factor. We suggest that Stonewall transcriptional regulation is essential in cystocytes for maturation into specialized nurse cells and oocyte.

Reference

    1. Altschul S. F.,
    2. Gish W.,
    3. Miller W.,
    4. Myers E. W.,
    5. Lipman D. J.
    (1990) Basic local alignment search tool. J. Mol. Biol 215, 403–410
    OpenUrlCrossRefPubMedWeb of Science
    1. Artavanis-Tsakonas S.,
    2. Matsuno K.,
    3. Fortini M. E.
    (1995) Notch signaling. Science 268, 225–232
    OpenUrlAbstract/FREE Full Text
    1. Benedyk M. J.,
    2. Mullen J. R.,
    3. DiNardo S.
    (1994) odd-paired: a zinc finger pair-rule protein required for the timely activation of engrailed and wingless in Drosophila embryos. Genes Dev 8, 105–117
    OpenUrlAbstract/FREE Full Text
    1. Berg C. A.,
    2. Spradling A. C.
    (1991) Studies on the rate and site-specificity of P element transposition. Genetics 127, 515–524
    OpenUrlAbstract/FREE Full Text
    1. Brown E. H.,
    2. King R. C.
    (1964) Studies on the events resulting in the formation of an egg chamber in Drosophilamelanogaster. Growth 28, 41–81
    OpenUrlPubMed
    1. Brown N. H.,
    2. Kafatos F. C.
    (1988) Functional cDNA libraries from Drosophila embryos. J. Mol. Biol 203, 425–437
    OpenUrlCrossRefPubMedWeb of Science
    1. Christerson L. B.,
    2. McKearin D. M.
    (1994) orb is required for anteroposterior and dorsoventral patterning during Drosophila oogenesis. Genes Dev 8, 614–628
    OpenUrlAbstract/FREE Full Text
    1. Church G. M.,
    2. Gilbert. W.
    (1984) Genomic sequencing. Proc. Natl. Acad. Sci. USA 81, 1991–1995
    OpenUrlAbstract/FREE Full Text
    1. Conaway R. C.,
    2. Conaway J. W.
    (1993) General initiation factors for RNA polymerase II. Ann. Rev. Biochem 62, 161–190
    OpenUrlCrossRefPubMedWeb of Science
    1. Cooley L.,
    2. Berg C.,
    3. Spradling A. C.
    (1988) Controlling P element insertional mutagenesis. Trends Genet 4, 254–258
    OpenUrlCrossRefPubMedWeb of Science
    1. Cooley L.,
    2. Verheyen E.,
    3. Ayers K.
    (1992) chickadee encodes a profilin required for intercellular cytoplasm transport during Drosophila oogenesis. Cell 69, 173–184
    OpenUrlCrossRefPubMedWeb of Science
    1. England B. P.,
    2. Heberlein U.,
    3. Tjian R.
    (1990) Purified Drosophila transcription factor, Adh distal factor-1 (Adf-1), binds to sites in several Drosophila promoters and activates transcription. J. Biol. Chem 265, 5086–5094
    OpenUrlAbstract/FREE Full Text
    1. England B. P.,
    2. Admon A.,
    3. Tjian R.
    (1992) Cloning of Drosophila transcription factor Adf-1 reveals homology to Myb oncoproteins. Proc. Natl. Acad. Sci. USA 89, 683–687
    OpenUrlAbstract/FREE Full Text
    1. Feinberg A. P.,
    2. Vogelstein B.
    (1984) Addendum, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analyt. Biochem 137, 266–267
    OpenUrlCrossRefPubMedWeb of Science
    1. Frampton J.,
    2. Gibson T. J.,
    3. Ness S. A.,
    4. Doderlein G.,
    5. Graf T.
    (1991) Proposed structure for the DNA-binding domain of the Myb oncoprotein based on model building and mutational analysis. Protein Engineering 4, 891–901
    OpenUrlAbstract/FREE Full Text
    1. Gavrieli Y.,
    2. Sherman Y.,
    3. Ben-Sasson S. A.
    (1992) Identification of programmed cell death via specific labeling of nuclear DNA fragmentation. J. Cell Biol 119, 493–501
    OpenUrlAbstract/FREE Full Text
    1. Gray S.,
    2. Szymanski P.,
    3. Levine M.
    (1994) Short-range repression permits multiple enhancers to function autonomously within a complex promoter. Genes Dev 8, 1829–1838
    OpenUrlAbstract/FREE Full Text
    1. Harrison S. C.,
    2. Aggarwal A. K.
    (1990) DNA recognition by proteins with the helix-turn-helix motif. Ann. Rev. Biochem 59, 933–969
    OpenUrlCrossRefPubMedWeb of Science
    1. Heberlein U.,
    2. England B.,
    3. Tjian R.
    (1985) Characterization of Drosophila transcription factors that activate the tandem promoters of the Alcohol Dehydrogenase gene. Cell 41, 965–977
    OpenUrlCrossRefPubMedWeb of Science
    1. Karpen G. H.,
    2. Spradling A. C.
    (1992) Analysis of subtelomeric heterochromatin in the Drosophila minichromosome Dp1187 by single P element insertional mutagenesis. Genetics 132, 737–753
    OpenUrlAbstract/FREE Full Text
    1. King R. C.,
    2. Storto P. D.
    (1988) The role of the otu gene in Drosophila oogenesis,. BioEssays 8, 18–24
    OpenUrlCrossRefPubMedWeb of Science
    1. Koch E. A.,
    2. Smith P. A.,
    3. King R. C.
    (1966) The division and differentiation of Drosophila cystocytes. J. Morphol 121, 55–70
    1. Lantz V.,
    2. Ambrosio L.,
    3. Schedl P.
    (1992) The Drosophila orb gene is predicted to encode sex-specific germline RNA-binding proteins and has localized transcripts in ovaries and early embryos. Development 115, 75–88
    OpenUrlAbstract
    1. Lantz V.,
    2. Chang J. S.,
    3. Horabin J. I.,
    4. Bopp D.,
    5. Schedl P.
    (1994) The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and estblishment of polarity. Genes Dev 8, 598–613
    OpenUrlAbstract/FREE Full Text
    1. Lin H.,
    2. Yue L.,
    3. Spradling A. C.
    (1994) The Drosophila fusome, a germline specific organelle, contains membrane skeletal proteins and functions in cyst formation. Development 120, 947–956
    OpenUrlAbstract
    1. Lin H.,
    2. Spradling A. C.
    (1995) Fusome asymmetry and oocyte determination in Drosophila. Dev. Genet 16, 6–12
    OpenUrlCrossRefPubMedWeb of Science
    1. Lundell M. J.,
    2. Hirsh J.
    (1994) A new visible light DNA fluorochrome for confocal microscopy. Biotechniques 16, 434–440
    OpenUrlPubMed
    1. McKearin D. M.,
    2. Spradling A. C.
    (1990) bag-of-marbles; A Drosophila gene required to initiate male and female gametogenesis. Genes Dev 4, 2242–2251
    OpenUrlAbstract/FREE Full Text
    1. McKearin D. M.,
    2. Ohlstein B.
    (1995) A role for the Drosophila Bag-of-marbles protein in the differentiation of cystoblasts from germline stem cells. Development 121, 2937–2947
    OpenUrlAbstract
    1. Mitchell P. J.,
    2. Tjian R.
    (1989) Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245, 371–378
    OpenUrlAbstract/FREE Full Text
    1. Oliver B.,
    2. Perrimon N.,
    3. Mahowald A. P.
    (1987) The ovo locus is required for sex-specific germ line maintenance in Drosophila. Genes Dev 1, 913–923
    OpenUrlAbstract/FREE Full Text
    1. Ran B.,
    2. Boop R.,
    3. Suter B.
    (1994) Null alleles reveal novel requirements for Bic-D during Drosophila oogenesis and zygotic development. Development 120, 1233–1242
    OpenUrlAbstract
    1. Roberts S. G. E.,
    2. Green M.
    (1994) Activator-induced conformational change in general transcription factor TFIIB. Nature 371, 717–720
    OpenUrlCrossRefPubMedWeb of Science
    1. Robertson H. M.,
    2. Preston C. R.,
    3. Philips R. W.,
    4. Johnson-Schlitz D. M.,
    5. Benz W. K.,
    6. Engels W. R.
    (1988) A stable genomic source ofPelement transposase in Drosophila melanogaster. Genetics 118, 461–470
    OpenUrlAbstract/FREE Full Text
    1. Rost B.,
    2. Sander C.,
    3. Schneider R.
    (1994) PHD--an automatic mail server for protein secondary structure prediction. Computer Applic. Biosciences 10, 53–60
    OpenUrlAbstract/FREE Full Text
    1. Schupbach T.,
    2. Roth S.
    (1994) Dorsoventral patterning in Drosophila oogenesis. Curr. Opin. Genet. Devel 4, 502–507
    OpenUrlCrossRefPubMed
    1. Schupbach T.,
    2. Wieschaus E. F.
    (1991) Female sterile mutations of the second chromosome of Drosophila melanogaster, II. Mutations blocking oogenesis or altering egg morphology. Genetics 129, 1119–1136
    OpenUrlAbstract/FREE Full Text
    1. Suter B.,
    2. Steward R.
    (1991) Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation. Cell 67, 917–926
    OpenUrlCrossRefPubMedWeb of Science
    1. Tautz D.,
    2. Pfeifle C.
    (1989) A non-radioactive in situ hybridization method for localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98, 81–85
    OpenUrlCrossRefPubMedWeb of Science
    1. Theurkauf W. E.,
    2. Alberts B. M.,
    3. Jan Y. N.,
    4. Jongens T. A.
    (1993) A central role for microtubles in the differentation of Drosophila oocytes. Development 118, 1169–1180
    OpenUrlAbstract/FREE Full Text
    1. Thummel C.,
    2. Pirrotta V.
    (1992) New pCaSpeR P element vectors. Dros. Inf. Serv 71, 150–.
    OpenUrl
    1. Wasserman S. A.
    (1993) A conserved signal transduction pathway regulating the activity of the rel-like proteins dorsal and NF-kappa B. Molec. Biol. Cell 4, 767–771
    OpenUrlFREE Full Text
    1. Yue L.,
    2. Spradling A.C.
    (1992) Hu-li tai shao, a gene required for ring canal formation during Drosophila oogenesis, encodes a homolog of adducin. Genes Dev 6, 2443–2454
    OpenUrlAbstract/FREE Full Text
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JOURNAL ARTICLES
The Drosophila stonewall gene encodes a putative transcription factor essential for germ cell development
K.A. Clark, D.M. McKearin
Development 1996 122: 937-950;
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