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JOURNAL ARTICLES
Sclerotome development and peripheral nervous system segmentation in embryonic zebrafish
E.M. Morin-Kensicki, J.S. Eisen
Development 1997 124: 159-167;
E.M. Morin-Kensicki
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J.S. Eisen
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Summary

Vertebrate embryos display segmental patterns in many trunk structures, including somites and peripheral nervous system elements. Previous work in avian embryos suggests a role for somite-derived sclerotome in segmental patterning of the peripheral nervous system. We investigated sclerotome development and tested its role in patterning motor axons and dorsal root ganglia in embryonic zebrafish. Individual somite cells labeled with vital fluorescent dye revealed that some cells of a ventromedial cell cluster within each somite produced mesenchymal cells that migrated to positions expected for sclerotome. Individual somites showed anterior/posterior distinctions in several aspects of development: (1) anterior ventromedial cluster cells produced only sclerotome, (2) individual posterior ventromedial cluster cells produced both sclerotome and muscle, and (3) anterior sclerotome migrated earlier and along a more restricted path than posterior sclerotome. Vital labeling showed that anterior sclerotome colocalized with extending identified motor axons and migrating neural crest cells. To investigate sclerotome involvement in peripheral nervous system patterning, we ablated the ventromedial cell cluster and observed subsequent development of peripheral nervous system elements. Primary motor axons were essentially unaffected by sclerotome ablation, although in some cases outgrowth was delayed. Removal of sclerotome did not disrupt segmental pattern or development of dorsal root ganglia or peripheral nerves to axial muscle. We propose that peripheral nervous system segmentation is established through interactions with adjacent paraxial mesoderm which develops as sclerotome in some vertebrate species and myotome in others.

REFERENCES

    1. Aoyama H.,
    2. Asamoto K.
    (1988) Determination of somite cells: independence of cell differentiation and morphogenesis. Development 104, 15–28
    OpenUrlAbstract
    1. Bagnall K. M.
    (1992) The migration and distribution of somite cells after labelling with the carbocyanine dye, DiI: the relationship of this distribution to segmentation in the vertebrate body. Anat. Embryol 185, 317–324
    OpenUrlPubMed
    1. Bagnall K. M.,
    2. Higgins S. J.,
    3. Sanders E. J.
    (1988) The contribution made by a single somite to the vertebral column: experimental evidence in support of resegmentation using the chick-quail chimaera model. Development 103, 69–85
    OpenUrlAbstract
    1. Bellairs R.
    (1963) The development of somites in the chick embryo. J. Embryol. exp. Morphol 11, 697–714
    1. Beresford B.
    (1983) Brachial muscles in the chick embryo: fate of individual somites. J. Embryol. exp. Morphol 77, 99–116
    OpenUrlPubMed
    1. Bronner-Fraser M.,
    2. Stern C. D.
    (1991) Effects of mesodermal tissues on avian neural crest migration. Dev. Biol 143, 213–217
    OpenUrlCrossRefPubMedWeb of Science
    1. Butcher E. O.
    (1929) The development of the somites in the white rat (Mus norvegieus albinus) and the fate of the myotomes, neural tube, and gut in the tail. Am. J. Anat 44, 381–439
    OpenUrlCrossRefWeb of Science
    1. Detwiler S. R.
    (1934) An experimental study of spinal nerve segmentation inamblystoma with reference to the plurisegmental contribution to the brachial plexus. J. Exp. Zool 67, 395–441
    OpenUrlCrossRefWeb of Science
    1. Devoto S. H.,
    2. Melançon E.,
    3. Eisen J. S.,
    4. Westerfield M.
    (1996) Identification of separate slow and fast muscle precursor cells in vivo, prior to somite formation. Development 122, 3371–3380
    OpenUrlAbstract
    1. Eisen J. S.
    (1994) Development of motoneuronal phenotype. Ann. Rev. Neurosci 17, 1–30
    OpenUrlPubMedWeb of Science
    1. Eisen J. S.,
    2. Myers P. Z.,
    3. Westerfield M.
    (1986) Pathway selection by growth cones of identified motoneurones in live zebrafish embryos. Nature 320, 269–271
    OpenUrlCrossRefPubMed
    1. Eisen J. S.,
    2. Pike S. H.
    (1991) The spt-1 mutation alters segmental arrangement and axonal development of identified neurons in the spinal cord of the embryonic zebrafish. Neuron 6, 767–776
    OpenUrlCrossRefPubMedWeb of Science
    1. Eisen J. S.,
    2. Pike S. H.,
    3. Debu B.
    (1989) The growth cones of identified motoneurons in embryonic zebrafish select appropriate pathways in the absence of specific cellular interactions. Neuron 2, 1097–1104
    OpenUrlCrossRefPubMedWeb of Science
    1. Halpern M. E.,
    2. Thisse C.,
    3. Ho R. K.,
    4. Thisse B.,
    5. Riggleman B.,
    6. Trevarrow B.,
    7. Weinberg E. S.,
    8. Postlethwait J. H.,
    9. Kimmel C. B.
    (1995) Cell-autonomous shift from axial to paraxial mesodermal development in zebrafish floating head mutants. Development 121, 4257–4264
    OpenUrlAbstract
    1. Hanneman E.,
    2. Westerfield M.
    (1989) Early expression of acetylcholinesterase activity in functionally distinct neurons of the zebrafish. J. Comp. Neurol 284, 350–361
    OpenUrlCrossRefPubMedWeb of Science
    1. Kaehn K.,
    2. Jacob H.J.,
    3. Christ B.,
    4. Hinrichsen K.,
    5. Poelmann R. F.
    (1988) The onset of myotome formation in the chick. Anat. Embryol 177, 191–201
    OpenUrlCrossRefPubMed
    1. Kalcheim C.,
    2. Teillet M. A.
    (1989) Consequences of somite manipulation on the pattern of dorsal root ganglion development. Development 106, 85–93
    OpenUrlAbstract
    1. Keynes R. J.,
    2. Stern C. D.
    (1984) Segmentation in the vertebrate nervous system. Nature 310, 786–789
    OpenUrlCrossRefPubMed
    1. Kimmel C. B.,
    2. Ballard W. W.,
    3. Kimmel S. R.,
    4. Ullmann B.,
    5. Schilling T. F.
    (1995) Stages of embryonic development in the zebrafish. Dev. Dyn 203, 253–310
    OpenUrlPubMedWeb of Science
    1. Lallier T. E.,
    2. Bronner-Fraser M.
    (1988) A spatial and temporal analysis of dorsal root and sympathetic ganglion formation in the avian embryo. Dev. Biol 127, 99–112
    OpenUrlCrossRefPubMedWeb of Science
    1. Lehmann F. E.
    (1927) Further studies on the morphogenetic role of the somites in the development of the nervous system of amphibians. J. Exp. Zool 49, 93–131
    OpenUrlCrossRefWeb of Science
    1. Lewis J.,
    2. Chevallier A.,
    3. Kieny M.,
    4. Wolpert L.
    (1981) Muscle nerve branches do not develop in chick wings devoid of muscle. J. Embryol. Exp. Morphol 64, 211–232
    OpenUrlPubMedWeb of Science
    1. Mauger A.
    (1972) Rôle du mesoderme somitique dans le developpement du plumage dorsal chez l'embryon de poulet. II. Regionalisation du mesoderme plumigene. J. Embryol. exp. Morphol 28, 343–366
    OpenUrlPubMedWeb of Science
    1. Metcalf W. K.
    (1985) Sensory neuron growth cones comigrate with posterior lateral line primordial cells in zebrafish. J. Comp. Neurol 238, 218–224
    OpenUrlCrossRefPubMedWeb of Science
    1. Myers P. Z.
    (1985) Spinal motoneurons of the larval zebrafish. J. Comp. Neurol 263, 555–561
    OpenUrl
    1. Myers P. Z.,
    2. Eisen J. S.,
    3. Westerfield M.
    (1986) Development and axonal outgrowth of identified motoneurons in the zebrafish. J. Neurosci 6, 2278–2289
    OpenUrlAbstract
    1. Neff A. W.,
    2. Malacinski G. M.,
    3. Chung H.-M.
    (1989) Amphibian (Urodele) myotomes display transitory anterior/posterior and medial/lateral differentiation patterns. Dev. Biol 132, 529–543
    OpenUrlCrossRefPubMed
    1. Norris W. E.,
    2. Stern C. D.,
    3. Keynes R. J.
    (1989) Molecular differences between the rostral and caudal halves of the sclerotome in the chick embryo. Development 105, 541–548
    OpenUrlAbstract/FREE Full Text
    1. Phelan K. A.,
    2. Hollyday M.
    (1990) Axon guidance in muscleless chickwings: the role of muscle cells in motoneuronal pathway selection and muscle nerve formation. J. Neurosci 10, 2699–2716
    OpenUrlAbstract
    1. Pike S. H.,
    2. Melancon E. F.,
    3. Eisen J. S.
    (1992) Pathfinding of zebrafish secondary motoneurons in the absence of normal pioneer axons. Development 114, 825–831
    OpenUrlAbstract
    1. Raible D. W.,
    2. Wood A.,
    3. Hodsdon W.,
    4. Henion P. D.,
    5. Weston J. A.,
    6. Eisen J. S.
    (1992) Segregation and early dispersal of neural crest cells in the embryonic zebrafish. Dev. Dynam 195, 29–42
    OpenUrlPubMedWeb of Science
    1. Rickmann M.,
    2. Fawcett J. W.,
    3. Keynes R. J.
    (1985) The migration of neural crest cells and the growth of motor axons through the rostral half of the chick somite. J. Embryol. Exp. Morphol 90, 437–455
    OpenUrlPubMedWeb of Science
    1. Stern C. D.,
    2. Keynes R. J.
    (1987) Interactions between somite cells: the formation and maintenance of segment boundaries in the chick embryo. Development 99, 261–272
    OpenUrlAbstract
    1. Tam P. P. L.,
    2. Trainor P. A.
    (1994) Specification and segmentation of the paraxial mesoderm. Anat. Embryol 189, 275–305
    OpenUrlPubMed
    1. Thisse C.,
    2. Thisse B.,
    3. Schilling T. F.,
    4. Postlethwait J. H.
    (1993) Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos. Development 119, 1203–1215
    OpenUrlAbstract
    1. Tosney K. W.
    (1987) Proximal tissues and patterned neurite outgrowth at the lumbosacral level of the chick embryo: deletion of the dermamyotome. Dev. Biol 122, 540–558
    OpenUrl
    1. Tosney K. W.
    (1988) Proximal tissues and patterned neurite outgrowth at the lumbosacral level of the chick embryo: partial and complete deletion of the somite. Dev. Biol 127, 266–286
    OpenUrlCrossRefPubMedWeb of Science
    1. Trevarrow B.,
    2. Marks D. L.,
    3. Kimmel C. B.
    (1990) Organization of hindbrain segments in the zebrafish embryo. Neuron 4, 669–679
    OpenUrlCrossRefPubMedWeb of Science
    1. Werner Y. L.
    (1971) The ontogenetic development of the vertebrae in some gekkonoid lizards. J. Morph 133, 41–92
    OpenUrlCrossRefPubMed
    1. Westerfield M.,
    2. McMurray J. V.,
    3. Eisen J. S.
    (1986) Identified motoneurons and their innervation of axial muscles in the zebrafish. J. Neurosci 6, 2267–2277
    OpenUrlAbstract
    1. Williams L. W.
    (1910) The somites of the chick. Am. J. Anat 2, 55–100
    OpenUrlCrossRef
    1. Youn B. W.,
    2. Malacinski G. M.
    (1981) Comparative analysis of amphibian somite morphogenesis: cell rearrangement patterns during rosette formation and myoblast fusion. J. Embryol. exp. Morphol 66, 1–26
    OpenUrlPubMed
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JOURNAL ARTICLES
Sclerotome development and peripheral nervous system segmentation in embryonic zebrafish
E.M. Morin-Kensicki, J.S. Eisen
Development 1997 124: 159-167;
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JOURNAL ARTICLES
Sclerotome development and peripheral nervous system segmentation in embryonic zebrafish
E.M. Morin-Kensicki, J.S. Eisen
Development 1997 124: 159-167;

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