Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in
  • Log out

Search

  • Advanced search
Development
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Development

  • Log in
Advanced search

RSS  Twitter  Facebook  YouTube 

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
JOURNAL ARTICLES
Retrospective clonal analysis of the cerebellum using genetic laacZ/lacZ mouse mosaics
L. Mathis, C. Bonnerot, L. Puelles, J.F. Nicolas
Development 1997 124: 4089-4104;
L. Mathis
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
C. Bonnerot
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
L. Puelles
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J.F. Nicolas
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & metrics
  • PDF
Loading

Summary

Analysis of lacZ neuronal clones in the mouse cerebellum demonstrates genealogical independence of the primary and secondary germinal epithelia (PGE and SGE) from early development. PGE precursors and their neuronal descendants are organised into two polyclonal groups of similar sizes that exhibit parasagittal patterning and generally respect the midline. The relationship between these two groups cannot be traced back in time to less than 80 independent cells, which were probably recruited following a period of non-coherent growth that distributes unrelated cells into distinct territories of the neural tube. A lateromedial clonal organisation is observed in the mature cerebellum, suggesting the existence of many small parasagittal domains of clonal restriction and/or of cell dispersion in the rostrocaudal but not in the mediolateral dimension. The organisation is orthogonal with respect to the cellular organisation in the neural tube as is the genetic organisation. Cellular and genetic patterning of the cerebellum therefore share similarities. A possible hypothesis is that distinct cell behaviours create the different clonal domains observed in this study and that the cellular and genetic organisation of the cerebellum are coordinated.

REFERENCES

    1. Altman J.
    (1972) Postnatal development of the cerebellar cortex in the rat. 3. Maturation of the components of the granular layer. J. Comp. Neurol 145, 465–513
    OpenUrlCrossRefPubMedWeb of Science
    1. Altman J.
    (1972) Postnatal development of the cerebellar cortex in the rat. I. The external germinal layer and the transitional molecular layer. J. Comp. Neurol 145, 353–97
    OpenUrlCrossRefPubMedWeb of Science
    1. Altman J.
    (1972) Postnatal development of the cerebellar cortex in the rat. II. Phases in the maturation of Purkinje cells and of the molecular layer. J. Comp. Neurol 145, 399–463
    OpenUrlCrossRefPubMedWeb of Science
    1. Altman J.,
    2. Bayer S. A.
    (1978) Prenatal development of the cerebellar system in the rat. I. Cytogenesis and histogenesis of the deep nuclei and the cortex of the cerebellum. J. Comp. Neurol 179, 23–48
    OpenUrlCrossRefPubMedWeb of Science
    1. Altman J.,
    2. Bayer S. A.
    (1985) Embryonic development of the rat cerebellum. I. Delineation of the cerebellar primordium and early cell movements. J. Comp. Neurol 231, 1–26
    OpenUrlCrossRefPubMedWeb of Science
    1. Altman J.,
    2. Bayer S. A.
    (1985) Embryonic development of the rat cerebellum. II. Translocation and regional distribution of the deep neurons. J. Comp. Neurol 231, 27–41
    OpenUrlCrossRefPubMedWeb of Science
    1. Altman J.,
    2. Bayer S. A.
    (1985) Embryonic development of the rat cerebellum. III. Regional differences in the time of origin, migration, and settling of Purkinje cells. J. Comp. Neurol 231, 42–65
    OpenUrlCrossRefPubMedWeb of Science
    1. Alvarez-Otero R.,
    2. Sotelo C.,
    3. Alvarado-Mallart R. M.
    (1993) Chick/quail chimeras with partial cerebellar grafts: an analysis of the origin and migration of cerebellar cells. J. Comp. Neurol 333, 597–615
    OpenUrlCrossRefPubMedWeb of Science
    1. Baader S. L.,
    2. Schilling M. L.,
    3. Rosengarten B.,
    4. Pretsch W.,
    5. Teutsch H. F.,
    6. Oberdick I.,
    7. Schilling K.
    (1996) Purkinje cell lineage and the topographic organization of the cerebellar cortex: a view from X inactivation mosaics. Dev. Biol 174, 393–406
    OpenUrlCrossRefPubMedWeb of Science
    1. Bally-Cuif L.,
    2. Wassef M.
    (1995) Determination events in the nervous system of the vertebrate embryo. Curr. Opin. Genet. Dev 5, 450–8
    OpenUrlCrossRefPubMedWeb of Science
    1. Crossley P. H.,
    2. Martin G. R.
    (1995) The mouse Fgf8 gene encodes afamily of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Development 121, 439–51
    OpenUrlAbstract
    1. Forss-Petter S.,
    2. Danielson P. E.,
    3. Catsicas S.,
    4. Battenberg E.,
    5. Price J.,
    6. Nerenberg M.,
    7. Stucliffe J. G.
    (1990) Transgenic mice expressiong-galactosidase in mature neurons under neuron-specific enolase promoter control. Neuron 5, 187–197
    OpenUrlCrossRefPubMedWeb of Science
    1. Fraser S.,
    2. Keynes R.,
    3. Lumsden A.
    (1990) Segmentation in the chick embryo hindbrain is defined by cell lineage restrictions. Nature 344, 431–435
    OpenUrlCrossRefPubMed
    1. Groves A. K.,
    2. George K. M.,
    3. Tissier S. J.,
    4. Engel J. D.,
    5. Brunet J. F.,
    6. Anderson D. J.
    (1995) Differential regulation of transcription factor gene expression and phenotypic markers in developing sympathetic neurons. Development 121, 887–901
    OpenUrlAbstract
    1. Guthrie S.,
    2. Muchamore I.,
    3. Kuroiwa A.,
    4. Marshall H.,
    5. Krumlauf R.,
    6. Lumsden A.
    (1992). Neuroectodermal autonomy of Hox-2.9 expression revealed by rhombomere transpositions. Nature 356, 157–9
    OpenUrlCrossRefPubMed
    1. Hallonet M. E.,
    2. LeDouarin N. M.
    (1993) Tracing neuroepithelial cells of the mesencephalic and metencephalic alar plates during cerebellar ontogeny in quail-chick chimaeras. Eur. J. Neurosci 5, 1145–55
    OpenUrlCrossRefPubMedWeb of Science
    1. Hallonet M. E. R.,
    2. Teillet M. A.,
    3. LeDouarin N. M.
    (1990) A new approach to the development of the cerebellum provided by the quail-chick marker system. Development 108, 19–31
    OpenUrlAbstract
    1. Hatten M. E.,
    2. Heintz N.
    (1995) Mechanisms of neural patterning and specification in the developing cerebellum. Annu Rev. Neurosci 18, 385–408
    OpenUrlCrossRefPubMedWeb of Science
    1. Hatten M. E.,
    2. Liem R. K.
    (1981) Astroglial cells provide a template for the positioning of developing cerebellar neurons in vitro. J. Cell Biol 90, 622–30
    OpenUrlAbstract/FREE Full Text
    1. Jennings C. G.
    (1988) What do chimearas tell us about cell lineages in the mammalian CNS?. Trends Neurosci 11, 46–9
    OpenUrlCrossRefPubMedWeb of Science
    1. Joyner A. L.
    (1996) Engrailed, Wnt and Pax genes regulate midbrain-hindbrain development. Trends Genet 12, 15–20
    OpenUrlCrossRefPubMedWeb of Science
    1. Kuratani S. C.,
    2. Eichele G.
    (1993) Rhombomere transplantation repatterns the segmental organization of cranial nerves and reveals cell-autonomous expression of a homeodomain protein. Development 117, 105–17
    OpenUrlAbstract/FREE Full Text
    1. LeDouarin N. M.
    (1969) Particularites du noyau interphasique chez la caille japonaise (Coturnix coturnix japonica). Utilisation de ces particularites comme marqueur biologique dans les recherches sur les interactions tissulaires et les migrations cellulaires au cours de l'ontogenese. Bull. Biol. Fr. Belg 103, 435–52
    OpenUrlPubMed
    1. Lumsden A.,
    2. Keynes R.
    (1989) Segmental patterns of neuronal development in the chick hindbrain. Nature 337, 424–428
    OpenUrlCrossRefPubMedWeb of Science
    1. Marin F.,
    2. Puelles L.
    (1995) Morphological fate of rhombomeres in quail/chick chimeras: a segmental analysis of hindbrain nuclei. Europ. J. Neurosci 7, 1714–38
    OpenUrlCrossRefPubMedWeb of Science
    1. Martinez S.,
    2. Alvarado-Mallart R. M.
    (1989) Rostral cerebellum originates from the caudal portion of the So-called ‘mesencephalic’ vesicle: a study using chick/quail chimeras. Europ. J. Neurosci 1, 549–560
    OpenUrlCrossRefPubMedWeb of Science
    1. McLaren A.
    (1972) Numerology of development. Nature 239, 274–6
    OpenUrlCrossRefPubMed
    1. McMahon A. P.,
    2. Joyner A. L.,
    3. Bradley A.,
    4. McMahon J. A.
    (1992). The midbrain-hindbrain phenotype of Wnt-1-/Wnt-1-mice results from stepwise deletion of engrailed-expressing cells by 9.5 days postcoitum. Cell 69, 581–95
    OpenUrlCrossRefPubMedWeb of Science
    1. Miale I. L.,
    2. Sidman R. L.
    (1961) An autoradiographic analysis of histogenesis in the mouse cerebellum. Exp. Neurol 4, 277–96
    OpenUrlCrossRefPubMedWeb of Science
    1. Millen K. J.,
    2. Hui C. C.,
    3. Joyner A. L.
    (1995) A role for En-2 and other murine homologues of Drosophila segment polarity genes in regulating positional information in the developing cerebellum. Development 121, 3935–45
    OpenUrlAbstract
    1. Millet S.,
    2. Bloch-Gallego E.,
    3. Simeone A.,
    4. Alvarado-Mallart R. M.
    (1996) The caudal limit of Otx2 gene expression as a marker of the midbain/hindbrain boundary: a study using in situ hybridization and chick/quail homotopic grafts. Development 122, 3785–97
    OpenUrlAbstract
    1. Mintz B.
    (1965) Genetic mosaicism in adult mice of quadriparental lineage. Science 148, 1232–1233
    OpenUrlAbstract/FREE Full Text
    1. Nicolas J. F.,
    2. Mathis L.,
    3. Bonnerot C.
    (1996) Evidence in the mouse for self-renewing stem cells in the formation of a segmented longitudinal structure, the myotome. Development 122, 2933–46
    OpenUrlAbstract
    1. Oberdick J.,
    2. Schilling K.,
    3. Smeyne R. J.,
    4. Corbin J. G.,
    5. Bocchiaro C.,
    6. Morgan J. I.
    (1993) Control of segment-like patterns of gene expression in the mouse cerebellum. Neuron 10, 1007–18
    OpenUrlCrossRefPubMedWeb of Science
    1. Rakic P.
    (1971) Neuron-glia relationship during granule cell migration indeveloping cerebellar cortex. A Golgi and electronmicroscopic study in Macacus Rhesus. J. Comp. Neurol 141, 283–312
    OpenUrlCrossRefPubMedWeb of Science
    1. Sanes J.,
    2. Rubenstein J.,
    3. Nicolas J.F.
    (1986) Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos. EMBO J 5, 3133–42
    OpenUrlPubMedWeb of Science
    1. Simon H.,
    2. Hornbruch A.,
    3. Lumsden A.
    (1995) Independent assignment of antero-posterior and dorso-ventral positional values in the developing chick hindbrain. Curr. Biol 5, 205–14
    OpenUrlCrossRefPubMedWeb of Science
    1. Sotelo C.,
    2. Wassef M.
    (1991) Cerebellar development: afferent organization and Purkinje cell heterogeneity. Philos. Trans R. Soc. Lond. B Biol. Sci 331, 307–13
    OpenUrlCrossRefPubMedWeb of Science
    1. Sundin O. H.,
    2. Eichele G.
    (1990) A homeo domain protein reveals the metameric nature of the developing chick hindbrain. Genes Dev 4, 1267–76
    OpenUrlAbstract/FREE Full Text
    1. Vogel M. W.,
    2. Herrup K.
    (1993) A theoretical and experimental examination of cell lineage relationship among cerebellar Purkinje cells in the mouse. Dev. Biol 156, 49–68
    OpenUrlCrossRefPubMedWeb of Science
    1. Wetts R.,
    2. Herrup K.
    (1982) Cerebellar purkinje cells are descended from a small number of progenitors committed during early development: quantitative analysis of lurcher chimeric mice. J. Neurosci 2, 1494–8
    OpenUrlAbstract
    1. Wilkinson D. G.
    (1993) Molecular mechanisms of segmental patterning in the vertebrate hindbrain and neural crest. BioEssays 15, 499–505
    OpenUrlCrossRefPubMedWeb of Science
    1. Wilkinson D. G.,
    2. Bhatt S.,
    3. Chavrier P.,
    4. Bravo R.,
    5. Charnay P.
    (1989) Segment-specific expression of a zinc-finger gene in the developing nervous system of the mouse. Nature 337, 461–4
    OpenUrlCrossRefPubMed
    1. Wingate R. J. T.,
    2. Lumsden A.
    (1996) Persistence of rhombomeric organisation in the postsegmental hindbrain. Development 122, 2143–52
    OpenUrlAbstract
    1. Wurst W.,
    2. Auerbach A. B.,
    3. Joyner A. L.
    (1994) Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum. Development 120, 2065–75
    OpenUrlAbstract
    1. Zhang L.,
    2. Goldman J. E.
    (1996) Generation of cerebellar interneurons from dividing progenitors in white matter. Neuron 16, 47–54
    OpenUrlCrossRefPubMedWeb of Science
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Development.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Retrospective clonal analysis of the cerebellum using genetic laacZ/lacZ mouse mosaics
(Your Name) has sent you a message from Development
(Your Name) thought you would like to see the Development web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
JOURNAL ARTICLES
Retrospective clonal analysis of the cerebellum using genetic laacZ/lacZ mouse mosaics
L. Mathis, C. Bonnerot, L. Puelles, J.F. Nicolas
Development 1997 124: 4089-4104;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
JOURNAL ARTICLES
Retrospective clonal analysis of the cerebellum using genetic laacZ/lacZ mouse mosaics
L. Mathis, C. Bonnerot, L. Puelles, J.F. Nicolas
Development 1997 124: 4089-4104;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Info & metrics
  • PDF

Related articles

Cited by...

More in this TOC section

  • A BAC transgenic analysis of the Mrf4/Myf5 locus reveals interdigitated elements that control activation and maintenance of gene expression during muscle development
  • Visualization and functional characterization of the developing murine cardiac conduction system
  • Indian hedgehog activates hematopoiesis and vasculogenesis and can respecify prospective neurectodermal cell fate in the mouse embryo
Show more JOURNAL ARTICLES

Similar articles

Other journals from The Company of Biologists

Journal of Cell Science

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Kathryn Virginia Anderson (1952-2020)

Developmental geneticist Kathryn Anderson passed away at home on 30 November 2020. Tamara Caspary, a former postdoc and friend, remembers Kathryn and her remarkable contribution to developmental biology.


Zooming into 2021

In a new Editorial, Editor-in-Chief James Briscoe and Executive Editor Katherine Brown reflect on the triumphs and tribulations of the last 12 months, and look towards a hopefully calmer and more predictable year.


Read & Publish participation extends worldwide

Over 60 institutions in 12 countries are now participating in our Read & Publish initiative. Here, James Briscoe explains what this means for his institution, The Francis Crick Institute. Find out more and view our full list of participating institutions.


Upcoming special issues

Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021

The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022

Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.


Development presents...

Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Sign up to join our next session:

10 February
Time: 13:00 (GMT)
Chaired by: preLights

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Sign up for alerts

About us

  • About Development
  • About the Node
  • Editors and board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • Biology Open transfer

Journal info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact Development
  • Subscriptions
  • Advertising
  • Feedback

 Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992