Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in
  • Log out

Search

  • Advanced search
Development
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Development

  • Log in
Advanced search

RSS  Twitter  Facebook  YouTube 

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
JOURNAL ARTICLES
nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation
I. Varlet, J. Collignon, E.J. Robertson
Development 1997 124: 1033-1044;
I. Varlet
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. Collignon
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
E.J. Robertson
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & metrics
  • PDF
Loading

Summary

Mouse nodal, a member of the TGFbeta family of secreted growth factors is essential for gastrulation. We recently generated a nodal(lacZ) reporter allele by homologous recombination in ES cells. In the present study, beta-galactosidase staining in the perigastrulation-stage embryo has demonstrated the site of highest nodal expression is localised to the prospective posterior region of the epiblast marking the site of primitive streak formation. We also documented transient nodal.lacZ expression in the visceral endoderm prior to and during early streak formation. A mosaic analysis using wild-type ES cells to rescue nodal-deficient embryos allowed us to document functionally distinct nodal activities in the embryonic ectodermal and primitive endodermal cell lineages. nodal signaling in the ectoderm is necessary for primitive streak formation as the gastrulation defect of nodal-deficient embryos can be rescued by the inclusion of small numbers of wild-type cells. In addition, we show that chimeric embryos composed of nodal-deficient primitive endoderm fail to develop rostral neural structures. Thus we conclude that the action of nodal, a TGFbeta-related growth factor expressed in the primitive endoderm, is critical for patterning of the anterior aspects of the A-P axis.

REFERENCES

    1. Acampora D.,
    2. Mazan S.,
    3. Lallemand Y.,
    4. Avantaggiato V.,
    5. Maury M.,
    6. Simeone A.,
    7. Brûlet P.
    (1995) Forebrain and midbrain regions are deleted in Otx2 mutants due to a defective anterior neuroectoderm specification during gastrulation. Development 121, 3279–3290
    OpenUrlAbstract
    1. Ang S.-L.,
    2. Conlon R. A.,
    3. Jin O.,
    4. Rossant J.
    (1994) Positive and negative signals from mesoderm regulate the expression of mouse Otx2 in ectoderm explants. Development 120, 2979–2989
    OpenUrlAbstract
    1. Ang S.-L.,
    2. Jin O.,
    3. Rhinn M.,
    4. Daigle N.,
    5. Stevenson L.,
    6. Rossant J.
    (1996) A targeted mouse Otx2 mutation leads to severe defects in gastrulation and formation of axial mesoderm and to deletion of rostral brain. Development 122, 243–252
    OpenUrlAbstract
    1. Ang S.-L.,
    2. Rossant J.
    (1993) Anterior mesendoderm induces mouse Engrailed genes in explant cultures. Development 118, 139–149
    OpenUrlAbstract
    1. Ang S.-L.,
    2. Rossant J.
    (1994) HNF-3 is essential for node and notochord formation in mouse development. Cell 78, 561–574
    OpenUrlCrossRefPubMedWeb of Science
    1. Azar Y.,
    2. Eyal-Giladi H.
    (1981) Interaction of epiblast and hypoblast in the formation of the primitive streak and the embryonic axis in chick, as revealed by hypoblast-rotation experiments. J. Embryol. Exp. Morph 61, 133–144
    OpenUrlPubMedWeb of Science
    1. Barnes J. D.,
    2. Crosby J. L.,
    3. Jones C. M.,
    4. Wright C. V. E.,
    5. Hogan B. L. M.
    (1994) Embryonic expression of Lim-1, the mouse homolog of Xenopus XLim-1, suggests a role in lateral mesoderm differentiation and neurogenesis. Dev. Biol 161, 168–178
    OpenUrlCrossRefPubMedWeb of Science
    1. Beddington R. S. P.
    (1994) Induction of a second neural axis by the mouse node. Development 120, 613–620
    OpenUrlAbstract
    1. Beddington R. S. P.,
    2. Robertson E. J.
    (1989) An assessment of the developmental potential of embryonic stem cells in the midgestation embyro. Development 105, 733–737
    OpenUrlAbstract/FREE Full Text
    1. Bouwmeester T.,
    2. Kim S.-H.,
    3. Sasai Y.,
    4. Lu B.,
    5. De Robertis E. M.
    (1996) Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer. Nature 382, 595–601
    OpenUrlCrossRefPubMed
    1. Collignon J.,
    2. Varlet I.,
    3. Robertson E. J.
    (1996) Relationship between asymmetric nodal expression and the direction of embryonic turning. Nature 381, 155–158
    OpenUrlCrossRefPubMed
    1. Conlon F. L.,
    2. Barth K. S.,
    3. Robertson E. J.
    (1991). A novel retrovirally-induced embryonic lethal mutation in the mouse; assessment of the developmental fate of ES cells homozygous for the 413.d proviral integration. Development 111, 969–981
    OpenUrlAbstract/FREE Full Text
    1. Conlon F. L.,
    2. Lyons K. M.,
    3. Takaesu N.,
    4. Barth K. S.,
    5. Kispert A.,
    6. Herrmann B.,
    7. Robertson E. J.
    (1994) A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse. Development 120, 1919–1928
    OpenUrlAbstract
    1. Crossley P. H.,
    2. Martin G. R.
    (1995) The mouse Fgf-8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Development 121, 439–451
    OpenUrlAbstract
    1. Davis C.A.,
    2. Joyner A. L.
    (1988) Expression patterns of the homeo box-containing genes En-1 and En-2 and the proto-oncogene int-1 diverge during mouse development. Genes Dev 2, 1736–1744
    OpenUrlAbstract/FREE Full Text
    1. Echelard Y.,
    2. Epstein D. J.,
    3. St-Jacques B.,
    4. Shen L.,
    5. Mohler J.,
    6. McMahon J. A.,
    7. McMahon A. P.
    (1993) Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell 75, 1417–1430
    OpenUrlCrossRefPubMedWeb of Science
    1. Friedrich G.,
    2. Soriano P.
    (1991) Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev 5, 1513–1523
    OpenUrlAbstract/FREE Full Text
    1. Hermesz E.,
    2. Mackem S.,
    3. Mahon K. A.
    (1996) Rpx: a novel anterior-restricted homeobox gene progressively activated in the prechordal plate, anterior neural plate and Rathke's pouch of the mouse. Development 122, 41–52
    OpenUrlAbstract
    1. Jones C. M.,
    2. Kuehn M. R.,
    3. Hogan B. L. M.,
    4. Smith J. C.,
    5. Wright C. V. E.
    (1995) Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation. Development 121, 3651–3662
    OpenUrlAbstract
    1. Lawson K. A.,
    2. Meneses J. J.,
    3. Pedersen R. A.
    (1991) Clonal analysis of epiblast fate during germ layer formation in the mouse embryo. Development 113, 891–911
    OpenUrlAbstract
    1. Lawson K. A.,
    2. Pedersen R. A.
    (1987) Cell fate, morphogenetic movement and population kinetics of embryonic endoderm at the time of germ layer formation in the mouse. Development 101, 627–652
    OpenUrlAbstract
    1. Levin M.,
    2. Johnson R. L.,
    3. Stern C. D.,
    4. Kuehn M.,
    5. Tabin C.
    (1995) A molecular pathway determining left-right asymmetry in chick embryogenesis. Cell 82, 803–814
    OpenUrlCrossRefPubMedWeb of Science
    1. Lowe L. A.,
    2. Supp D. M.,
    3. Sampath K.,
    4. Yokoyama T.,
    5. Wright C. V. E.,
    6. Potter S. S.,
    7. Overbeek P.,
    8. Kuehn M. R.
    (1996) Conserved left-right asymmetry of nodal expression and alterations in murine situs inversus. Nature 381, 158–161
    OpenUrlCrossRefPubMedWeb of Science
    1. Matsuo I.,
    2. Kuratani S.,
    3. Kimura C.,
    4. Takeda N.,
    5. Aizawa S.
    (1995) Mouse Otx2 functions in the formation and patterning of rostral head. Genes Dev 9, 2646–2658
    OpenUrlAbstract/FREE Full Text
    1. Mitrani E.,
    2. Eyal-Giladi H.
    (1981) Hypoblastic cells can form a disk inducing an embryonic axis in the chick epiblast. Nature 289, 800–802
    OpenUrlCrossRefPubMed
    1. Monaghan A. P.,
    2. Kaestner K. H.,
    3. Grau E.,
    4. Schutz G.
    (1993) Postimplantation expression patterns indicate a role for the mouse forkhead /HNF-3, andgenes in determination of the definitive endoderm, chordamesoderm and neuroectoderm. Development 119, 567–578
    OpenUrlAbstract/FREE Full Text
    1. Placzek M.,
    2. Jessell T. M.,
    3. Dodd J.
    (1993) Induction of floor plate differentiation by contact-dependent, homeogenetic signals. Development 117, 205–218
    OpenUrlAbstract/FREE Full Text
    1. Poelmann R. E.
    (1981) The head process and the formation of definitive endoderm in the mouse embryo. Anat. Embryol 162, 41–49
    OpenUrlCrossRefPubMed
    1. Rosenquist T. A.,
    2. Martin G. R.
    (1995) Visceral endoderm-1 (VE-1): an antigen marker that distinguishes anterior from posterior embryonic visceral endoderm in the early post-implantation embryo. Mech. Dev 49, 117–121
    OpenUrlCrossRefPubMed
    1. Shawlot W.,
    2. Behringer R. R.
    (1995) Requirement for Lim1 in head-organizer function. Nature 374, 425–430
    OpenUrlCrossRefPubMed
    1. Shimamura K.,
    2. Hartigan D. J.,
    3. Martinez S.,
    4. Puelles L.,
    5. Rubenstein L. R.
    (1995) Longitudinal organization of the anterior neural plate and neural tube. Development 121, 3923–3933
    OpenUrlAbstract
    1. Smith W. C.,
    2. McKendry R.,
    3. Ribisi S., Jr.,
    4. Harland R. M.
    (1995) A nodal -related gene defines a physical and functional domain within the Spemann organizer. Cell 82, 37–46
    OpenUrlCrossRefPubMedWeb of Science
    1. Sulik K.,
    2. Dehart D. B.,
    3. Inagaki T.,
    4. Vrablic J. L.,
    5. Gesteland K.,
    6. Schoenwolf G. C.
    (1994) Morphogenesis of the murine node and notochordal plate. Dev. Dynamics 201, 260–278
    OpenUrlPubMedWeb of Science
    1. Thomas P.,
    2. Beddington R. S. P.
    (1996) Anterior primitive endoderm may be responsible for patterning the anterior neural plate in the mouse embryo. Current Biol 6, 1487–1496
    OpenUrlCrossRefPubMedWeb of Science
    1. Waddington C. H.
    (1933) Induction by the primitive streak and its derivatives in the chick. J. Exp. Biol 10, 38–46
    OpenUrlAbstract
    1. Weinstein D. C.,
    2. Ruiz i Altaba A.,
    3. Chen W. S.,
    4. Hoodless P.,
    5. Prezioso V. R.,
    6. Jessell T. M.,
    7. Darnell J. E., Jr
    (1994) The winged-helixtranscription factor HNF-3 is required for notochord development in the mouse embryo. Cell 78, 575–588
    OpenUrlCrossRefPubMedWeb of Science
    1. Wilkinson D. G.,
    2. Bhatt S.,
    3. Chavrier P.,
    4. Bravo R.,
    5. Charnay P.
    (1989) Segment-specific expression of a zinc-finger gene in the developing nervous system of the mouse. Nature 337, 461–464
    OpenUrlCrossRefPubMed
    1. Zhou X.,
    2. Sasaki H.,
    3. Lowe L.,
    4. Hogan B. L. M.,
    5. Kuehn M. R.
    (1993) Nodal is a novel TGF--like gene expressed in the mouse node during gastrulation. Nature 361, 543–547
    OpenUrlCrossRefPubMed
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Development.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation
(Your Name) has sent you a message from Development
(Your Name) thought you would like to see the Development web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
JOURNAL ARTICLES
nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation
I. Varlet, J. Collignon, E.J. Robertson
Development 1997 124: 1033-1044;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
JOURNAL ARTICLES
nodal expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation
I. Varlet, J. Collignon, E.J. Robertson
Development 1997 124: 1033-1044;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Info & metrics
  • PDF

Related articles

Cited by...

More in this TOC section

  • Morphogenetic cell movements in the middle region of the dermomyotome dorsomedial lip associated with patterning and growth of the primary epaxial myotome
  • Germline and developmental roles of the nuclear transport factor importin (α)3 in C. elegans
  • Monofocal origin of telencephalic oligodendrocytes in the anterior entopeduncular area of the chick embryo
Show more JOURNAL ARTICLES

Similar articles

Other journals from The Company of Biologists

Journal of Cell Science

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Kathryn Virginia Anderson (1952-2020)

Developmental geneticist Kathryn Anderson passed away at home on 30 November 2020. Tamara Caspary, a former postdoc and friend, remembers Kathryn and her remarkable contribution to developmental biology.


Zooming into 2021

In a new Editorial, Editor-in-Chief James Briscoe and Executive Editor Katherine Brown reflect on the triumphs and tribulations of the last 12 months, and look towards a hopefully calmer and more predictable year.


Read & Publish participation extends worldwide

Over 60 institutions in 12 countries are now participating in our Read & Publish initiative. Here, James Briscoe explains what this means for his institution, The Francis Crick Institute. Find out more and view our full list of participating institutions.


Upcoming special issues

Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021

The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022

Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.


Development presents...

Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Sign up to join our next session:

10 February
Time: 13:00 (GMT)
Chaired by: preLights

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Sign up for alerts

About us

  • About Development
  • About the Node
  • Editors and board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • Biology Open transfer

Journal info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact Development
  • Subscriptions
  • Advertising
  • Feedback

 Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992