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JOURNAL ARTICLES
Genetic control of branching pattern and floral identity during Petunia inflorescence development
E. Souer, A. van der Krol, D. Kloos, C. Spelt, M. Bliek, J. Mol, R. Koes
Development 1998 125: 733-742;

Summary

A main determinant of inflorescence architecture is the site where floral meristems are initiated. We show that in wild-type Petunia bifurcation of the inflorescence meristem yields two meristems of approximately equal size. One terminates into a floral meristem and the other maintains its inflorescence identity. By random transposon mutagenesis we have generated two mutants in which the architecture of the inflorescence is altered. In the extra petals- (exp) mutant the inflorescence terminates with the formation of a single terminal flower. Phenotypic analysis showed that exp is required for the bifurcation of inflorescence meristems. In contrast, the aberrant leaf and flower- (alf) mutant is affected in the specification of floral meristem identity while the branching pattern of the inflorescence remains unaltered. A weak alf allele was identified that, after bifurcation of the inflorescence meristem, yields a ‘floral’ meristem with partial inflorescence characteristics. By analysing independent transposon dTph1 insertion alleles we show that the alf locus encodes the Petunia FLORICAULA/LEAFY homolog. In situ hybridisation shows that alf is expressed in the floral meristem and also in the vegetative meristem. Differences and similarities between these Petunia mutants and mutations affecting inflorescence architecture in other species will be discussed.

REFERENCES

    1. Allen K. D.,
    2. Sussex I. M.
    (1996). Falsiflora and anantha control early stages of floral meristem development in tomato (Lycopersiconesculentum Mill.). Planta 200, 254–264
    OpenUrl
    1. Angenent G. C.,
    2. Franken J.,
    3. Busscher M.,
    4. van Dijken A.,
    5. van Went J. L.,
    6. Dons H. J. M.,
    7. van Tunen A.J.
    (1995) A novel class of MADS box genes is involved in ovule development in Petunia. Plant Cell 7, 1569–1582
    OpenUrlAbstract/FREE Full Text
    1. Bradley D.,
    2. Carpenter R.,
    3. Copsey L.,
    4. Vincent C.,
    5. Rothstein S.,
    6. Coen E.
    (1996) Control of inflorescence architecture in Antirrhinum. Nature 379, 791–797
    OpenUrlCrossRefPubMed
    1. Bradley D.,
    2. Ratcliffe O.,
    3. Vincent C.,
    4. Carpenter R.,
    5. Coen E.
    (1997) Inflorescence commitment and architecture in Arabidopsis. Science 275, 80–83
    OpenUrlAbstract/FREE Full Text
    1. Cañas L. A.,
    2. Busscher M.,
    3. Angenent G. C.,
    4. Beltrán J.-P.,
    5. van Tunen A. J.
    (1994) Nuclear localization of the Petunia MADS box protein FBP1. Plant J 6, 597–604
    OpenUrlCrossRefWeb of Science
    1. Carpenter R.,
    2. Coen E. S.
    (1995) Transposon induced chimeras show that floricaula, a meristem identity gene, acts non-autonomously between cell layers. Development 121, 19–26
    OpenUrlAbstract
    1. Carpenter R.,
    2. Copsey L.,
    3. Vincent C.,
    4. Doyle S.,
    5. Magrath R.,
    6. Coen E.
    (1995) Control of flower development and phyllotaxy by meristem identity genes in Antirrhinum. Plant Cell 7, 2001–2011
    OpenUrlAbstract/FREE Full Text
    1. Coen E. S.,
    2. Roemro J. M.,
    3. Doyle S.,
    4. Elliott R.,
    5. Murphy G.,
    6. Carpenter R.
    (1990) floricaula: A homeotic gene required for flower development in Antirrhinum majus. Cell 63, 1311–1322
    OpenUrlCrossRefPubMedWeb of Science
    1. de Vetten N.,
    2. Quattrocchio F.,
    3. Mol J.,
    4. Koes R.
    (1997) The an11 locus controlling flower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast, plants, and animals. Genes Dev 11, 1422–1434
    OpenUrlAbstract/FREE Full Text
    1. Doodeman M.,
    2. Gerats A. G. M.,
    3. Schram A. W.,
    4. de Vlaming P.,
    5. Bianchi F.
    (1984) Genetic analysis of instability in Petuniahybrida 2. Unstable mutations at different loci as the result of transpositions of the genetic element inserted at the An1 locus. Theor. Appl. Genet 67, 357–366
    OpenUrlCrossRef
    1. Gerats A. G. M.,
    2. Kaye C.,
    3. Collins C.,
    4. Malmberg R. L.
    (1988) Polyamine levels in Petunia genotypes with normal and abnormal floral morphologies. Plant Physiol 86, 390–393
    OpenUrlAbstract/FREE Full Text
    1. Hantke S. S.,
    2. Carpenter R.,
    3. Coen E. S.
    (1995) Expression of floricaula in single cell layers of periclinal chimeras activates downstream homeotic genes in all layers of floral meristems. Development 121, 27–35
    OpenUrlAbstract
    1. Hofer J.,
    2. Turner L.,
    3. Hellens R.,
    4. Ambrose M.,
    5. Matthews P.,
    6. Michael A.,
    7. Ellis N.
    (1997) UNIFOLIATA regulates leaf and flower morphogenesis in pea. Curr. Biol 7, 581–587
    OpenUrlCrossRefPubMedWeb of Science
    1. Kelly A. J.,
    2. Bonnlander M. B.,
    3. Meeks-Wagner D. R.
    (1995) NFL, the tobacco homolog of FLORICAULA and LEAFY, is transcriptionally expressed in both vegetative and floral meristems. Plant Cell 7, 225–234
    OpenUrlAbstract/FREE Full Text
    1. Koes R.,
    2. Souer E.,
    3. van Houwelingen A.,
    4. Mur L.,
    5. Spelt C.,
    6. Quattrocchio F.,
    7. Wing J.,
    8. Oppedijk B.,
    9. Ahmed S.,
    10. Maes T.,
    11. Gerats T.,
    12. Hoogeveen P.,
    13. Meesters M.,
    14. Kloos D.,
    15. Mol J. N. M.
    (1995) Targeted gene inactivation in Petunia by PCR-based selection of transposon insertion mutants. Proc. Natl. Acad. Sci. USA 92, 8149–8153
    OpenUrlAbstract/FREE Full Text
    1. Napoli C. A.,
    2. Ruehle J.
    (1996) New mutations affecting meristem growth and potential in Petunia hybrida Vilm. J. Hered 87, 371–377
    OpenUrlAbstract/FREE Full Text
    1. Poteau S.,
    2. Nichols D.,
    3. Tooke F.,
    4. Coen E.,
    5. Batty N.
    (1997) The induction and maintenance of flowering in Impatiens. Development 124, 3343–3351
    OpenUrlAbstract
    1. Souer E.,
    2. van Houwelingen A.,
    3. Kloos D.,
    4. Mol J.,
    5. Koes R.
    (1996) The no apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries. Cell 85, 159–170
    OpenUrlCrossRefPubMedWeb of Science
    1. Tucker S. C.
    (1989) Overlapping organ initiation and common primordia in flowers of Pisum sativum (Leguminosae: Papilionoideae). Amer. J. Bot 76, 714–729
    OpenUrlCrossRefWeb of Science
    1. van der Krol A. R.,
    2. Brunelle A.,
    3. Tsuchimoto s.,
    4. Chua N.-H.
    (1993) Functional analysis of Petunia floral homeotic MADS-box gene pMADS1. Genes Dev 7, 1214–1228
    OpenUrlAbstract/FREE Full Text
    1. van Hoof A.,
    2. Green P. J.
    (1996) Premature nonsense codons decrease the stability of phytohemagglutinin mRNA in a position-dependent manner. Plant J 10, 415–424
    OpenUrlCrossRefPubMedWeb of Science
    1. Weigel D.,
    2. Alvarez J.,
    3. Smyth D. R.,
    4. Yanofsky M. F.,
    5. Meyerowitz E. M.
    (1992) LEAFY controls floral meristem identity in Arabidopsis. Cell 69, 843–860
    OpenUrlCrossRefPubMedWeb of Science
    1. Weigel D.
    (1995) The genetics of flower development: From floral induction to ovule morphogenesis. Annu. Rev. Genet 29, 19–39
    OpenUrlCrossRefPubMedWeb of Science
    1. Weigel D.,
    2. Nilsson O.
    (1995) A developmental switch sufficient for flower initiation in diverse plants. Nature 377, 495–500
    OpenUrlCrossRefPubMed
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JOURNAL ARTICLES
Genetic control of branching pattern and floral identity during Petunia inflorescence development
E. Souer, A. van der Krol, D. Kloos, C. Spelt, M. Bliek, J. Mol, R. Koes
Development 1998 125: 733-742;
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