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The role of Pax6 in restricting cell migration between developing cortex and basal ganglia
P. Chapouton, A. Gartner, M. Gotz
Development 1999 126: 5569-5579;

Summary

It is not clear to what extent restricted cell migration contributes to patterning of the developing telencephalon, since both restricted and widespread cell migration have been observed. Here, we have analysed dorso-ventral cell migration in the telencephalon of Pax6 mutant mice (Small Eye). The transcription factor Pax6 is expressed in the dorsal telencephalon, the cerebral cortex. Focal injections of adenoviral vectors containing the green fluorescent protein were used to follow and quantify cell movements between two adjacent regions in the developing telencephalon, the cerebral cortex and the ganglionic eminence (the prospective basal ganglia). The analysis in wild-type mice confirmed that the cortico-striatal boundary acts as a semipermeable filter and allows a proportion of cells from the ganglionic eminence to invade the cortex, but not vice versa. Ventro-dorsal cell migration was strongly enhanced in the Pax6 mutant. An essential function of Pax6 in the regionalisation of the telencephalon is then to limit the invasion of the cortex by cells originating in the ganglionic eminence. Cortical cells, however, remain confined to the cortex in the Pax6 mutant. Thus, dorsal and ventral cells are restricted to their respective territories by distinct mechanisms.

Reference

    1. Anderson S. A.,
    2. Eisenstat D. D.,
    3. Shi L.,
    4. Rubenstein J. L. R.
    (1997) Interneuron migration from basal forebrain to neocortex: dependence on DIx genes. Science 278, 474–476
    OpenUrlAbstract/FREE Full Text
    1. D.,
    2. Gooday D.,
    3. Hill R. E.,
    4. McConnell S. K.,
    5. Price D. J.
    (1997) Determination of the migratory capacity of embryonic cortical cells lacking the transcription factor Pax-6. Development 124, 5087–5096
    OpenUrlAbstract
    1. Casarosa S.,
    2. Fode C.,
    3. Guillemot F.
    (1999) Mash1 regulates neurogenesis in the ventral telencephalon. Development 126, 525–534
    OpenUrlAbstract
    1. Cooper J. A.
    (1987) Effects of cytochalasin and phalloidin on actin. J. Cell Biol 105, 1473–1478
    OpenUrlFREE Full Text
    1. Deacon T. W.,
    2. Pakzaban P.,
    3. Isacson O.
    (1994) The lateral ganglionic eminence is the origin of cells committed to striatal phenotypes: neural transplantation and developmental evidence. Brain Res 668, 211–219
    OpenUrlCrossRefPubMedWeb of Science
    1. DeCarlos J. A.,
    2. Lopez-Mascaraque L.,
    3. Valverde F.
    (1996) Dynamics of cell migration from the lateral ganglionic eminence in the rat. J. Neurosci 16, 6146–6156
    OpenUrlAbstract/FREE Full Text
    1. DeDiego I.,
    2. Smith-Fernández A.,
    3. Fairen A.
    (1994) Cortical cells that migrate beyond area boundaries: characterization of an early neuronal population in the lower intermediate zone of prenatal rats. Eur. J. Neurosci 6, 983–997
    OpenUrlCrossRefPubMedWeb of Science
    1. Figdor M. C.,
    2. Stern C. D.
    (1993) Segmental organisation of embryonic diencephalon. Nature 363, 630–634
    OpenUrlCrossRefPubMed
    1. Fishell G.,
    2. Mason C. A.,
    3. Hatten M. E.
    (1993) Dispersion of neural progenitors within the germinal zones of the forebrain. Nature 362, 636–638
    OpenUrlCrossRefPubMed
    1. Götz M.,
    2. Williams B. P.,
    3. Bolz J.,
    4. Price J.
    (1995) The specification of neuronal fate: A common precursor for neurotransmitter subtypes in the rat cerebral cortex in vitro. Eur. J. Neurosci 7, 889–898
    OpenUrlCrossRefPubMedWeb of Science
    1. Götz M.,
    2. Wizenmann A.,
    3. Reinhard S.,
    4. Lumsden A.,
    5. Price J.
    (1996) Selective adhesion of cells from different telencephalic regions. Neuron 16, 551–564
    OpenUrlCrossRefPubMedWeb of Science
    1. Götz M.,
    2. Stoykova A.,
    3. Gruss P.
    (1998) Pax6 controls radial glia differentiation in the cerebral cortex. Neuron 21, 1031–1044
    OpenUrlCrossRefPubMedWeb of Science
    1. Guthrie S.,
    2. Prince V.,
    3. Lumsden A.
    (1993) Selective dispersal of avian rhombomere cells in orthotopic and heterotopic grafts. Development 118, 527–538
    OpenUrlAbstract
    1. Hill R. E.,
    2. Favor J.,
    3. Hogan B. L. M.,
    4. Ton C. C. T.,
    5. Saunders G. F.,
    6. Hanson I. M.,
    7. Prosser J.,
    8. Jordan T.,
    9. Hastie N. D.,
    10. van Heyningen V.
    (1991) Mouse Small eye results from mutations in a paired-like homeobox-containing gene. Nature 354, 522–525
    OpenUrlCrossRefPubMed
    1. Inoue T.,
    2. Chisaka O.,
    3. Matsunami H.,
    4. Takeichi M.
    (1997) Cadherin-6 expression transiently delineates specific rhombomeres, other neural tube subdivisions, and neural crest subpopulations in mouse embryos. Dev. Biol 183, 183–194
    OpenUrlCrossRefPubMedWeb of Science
    1. Kawano H.,
    2. Fukuda T.,
    3. Kubo K.,
    4. Horie M.,
    5. Uyemura K.,
    6. Takeuchi K.,
    7. Osumi N.,
    8. Eto K.,
    9. Kawamura K.
    (1999) Pax6 is required for thalamocortical pathway formation in fetal rats. J. comp. Neurol 408, 147–160
    OpenUrlCrossRefPubMedWeb of Science
    1. Lavdas A. A.,
    2. Grigoriou M.,
    3. Pachnis V.,
    4. Parnavelas J. G.
    (1999) The medial ganglionic eminence gives rise to a population of early neurons in the developing cerebral cortex. J. Neurosci 19, 7881–7888
    OpenUrlAbstract/FREE Full Text
    1. Lumsden A.,
    2. Krumlauf R.
    (1996) Patterning the vertebrate neuraxis. Science 274, 1109–1114
    OpenUrlAbstract/FREE Full Text
    1. Lumsden A.,
    2. Gulisano M.
    (1997) Neocortical neurons: where do they come from?. Science 278, 402–404
    OpenUrlAbstract/FREE Full Text
    1. Matsunami H.,
    2. Takeichi M.
    (1995) Fetal brain subdivisions defined by R-and E-cadherin expressions: evidence for the role of cadherin activity in region-specific, cell-cell adhesion. Dev. Biol 172, 446–478
    OpenUrl
    1. Neyt C.,
    2. Welch M.,
    3. Langston A.,
    4. Kohtz J.,
    5. Fishell G.
    (1997) A short-range signal restricts cell movement between telencephalic proliferative zones. J. Neurosci 17, 9194–9203
    OpenUrlAbstract/FREE Full Text
    1. Nittenberg R.,
    2. Patel K.,
    3. Joshi Y.,
    4. Krumlauf R.,
    5. Wilkinson D. G.,
    6. Brickell P. M.,
    7. Tickle C.,
    8. Clarke J. D. W.
    (1997) Cell movements, neuronal organisation and gene expression in hindbrains lacking morphological boundaries. Development 124, 2297–2306
    OpenUrlAbstract
    1. Olsson M.,
    2. Campbell K.,
    3. Wictorin K.,
    4. Björklund A.
    (1995) Projection neurons in fetal striatal transplants are predominantly derived from the lateral ganglionic eminence. Neurosci 69, 1169–1182
    OpenUrlCrossRefPubMedWeb of Science
    1. O'Rourke N. A.,
    2. Chenn A.,
    3. McConnell S. K.
    (1997) Postmitotic neurons migrate tangentially in the cortical ventricular zone. Development 124, 997–1005
    OpenUrlAbstract
    1. Puelles L.,
    2. Rubenstein J. L. R.
    (1993) Expression patterns of homeobox and other putatitive regulatory genes in the embryonic mouse forebrain suggests a neuromeric organisation. Trends Neurosci 16, 472–479
    OpenUrlCrossRefPubMedWeb of Science
    1. Stoykova A.,
    2. Fritsch R.,
    3. Walther C.,
    4. Gruss P.
    (1996) Forebrain patterning defects in Small eye mutant mice. Development 122, 3453–3465
    OpenUrlAbstract
    1. Stoykova A.,
    2. Götz M.,
    3. Gruss P.,
    4. Price J.
    (1997) Pax6-dependent regulation of adhesive patterning, R-cadherin expression and boundary formation in developing forebrain. Development 124, 3765–3777
    OpenUrlAbstract
    1. Sussel L.,
    2. Marin O.,
    3. Kimura S.,
    4. Rubenstein J. L. R.
    (1999). Loss of Nkx2.1 homeobox gene function results in ventral to dorsal molecular respecification within the basal telencephalon: evidence for a transformation of the pallidum into the striatum. Development 126, 3359–3370
    OpenUrlAbstract
    1. Tamamaki N.,
    2. Fujimori K. E.,
    3. Takauji R.
    (1997) Origin and route of tangentially migrating neurons in the developing neocortical intermediate zone. J. Neurosci 17, 8313–8323
    OpenUrlAbstract/FREE Full Text
    1. Xu Q.,
    2. Mellitzer G.,
    3. Robinson V.,
    4. Wilkinson D. G.
    (1999) In vivo cell sorting in complementary segmental domains mediated by Eph receptors and ephrins. Nature 399, 267–271
    OpenUrlCrossRefPubMed
    1. Zhu Y.,
    2. Li H.,
    3. Zhou L.,
    4. Wu J. Y.,
    5. Rao Y.
    (1999) Cellular and molecular guidance of GABAergic neuronal migration from an extracortical origin to the neocortex. Neuron 23, 473–485
    OpenUrlCrossRefPubMedWeb of Science
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JOURNAL ARTICLES
The role of Pax6 in restricting cell migration between developing cortex and basal ganglia
P. Chapouton, A. Gartner, M. Gotz
Development 1999 126: 5569-5579;
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