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JOURNAL ARTICLES
The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
K. Lynn, A. Fernandez, M. Aida, J. Sedbrook, M. Tasaka, P. Masson, M.K. Barton
Development 1999 126: 469-481;

Summary

Several lines of evidence indicate that the adaxial leaf domain possesses a unique competence to form shoot apical meristems. Factors required for this competence are expected to cause a defect in shoot apical meristem formation when inactivated and to be expressed or active preferentially in the adaxial leaf domain. PINHEAD, a member of a family of proteins that includes the translation factor eIF2C, is required for reliable formation of primary and axillary shoot apical meristems. In addition to high-level expression in the vasculature, we find that low-level PINHEAD expression defines a novel domain of positional identity in the plant. This domain consists of adaxial leaf primordia and the meristem. These findings suggest that the PINHEAD gene product may be a component of a hypothetical meristem forming competence factor. We also describe defects in floral organ number and shape, as well as aberrant embryo and ovule development associated with pinhead mutants, thus elaborating on the role of PINHEAD in Arabidopsis development. In addition, we find that embryos doubly mutant for PINHEAD and ARGONAUTE1, a related, ubiquitously expressed family member, fail to progress to bilateral symmetry and do not accumulate the SHOOT MERISTEMLESS protein. Therefore PINHEAD and ARGONAUTE1 together act to allow wild-type growth and gene expression patterns during embryogenesis.

Reference

    1. Barton M.K.,
    2. Poethig S.
    (1993) Formation of the shoot apical meristem in Arabidopsisthaliana: an analysis of development in the wild type and in the shootmeristemless mutant. Development 119, 823–831
    OpenUrlAbstract/FREE Full Text
    1. Bohmert K.,
    2. Camus I.,
    3. Bellini C.,
    4. Bouchez D.,
    5. Caboche M.,
    6. Benning C.
    (1997) AGO1 defines a novel locus of Arabidopsis controlling leaf development. EMBO J 17, 170–180
    OpenUrlAbstract
    1. Chuck G.,
    2. Lincoln C.,
    3. Hake S.
    (1996) KNAT1 induces lobed leaves with ectopic meristems when overexpressed in Arabidopsis. Plant Cell 8, 1277–1289
    OpenUrlAbstract/FREE Full Text
    1. ndrizzi K.,
    2. Moussian B.,
    3. Haecker A.,
    4. Levin J.Z.,
    5. Laux T.
    (1996) The SHOOTMERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE. PlantJ 10, 967–979
    OpenUrlCrossRefPubMedWeb of Science
    1. Estruch J.J.,
    2. Prinsen E.,
    3. Onckelen H.V.,
    4. Schell J.,
    5. Spena A.
    (1991) Viviparous leaves produced by somatic activation of an inactive cytokinin-synthesizing gene. Science 254, 1364–1367
    OpenUrlAbstract/FREE Full Text
    1. Irish V.,
    2. Sussex I.M.
    (1992) A fate map of the Arabidopsis embryonic shoot apical meristem. Development 115, 745–753
    OpenUrlAbstract/FREE Full Text
    1. Long J.A.,
    2. Moan E.I.,
    3. Medford J.,
    4. Barton M.K.
    (1996) A member of the KNOTTED class of homeodomain proteins encoded by the SHOOTMERISTEMLESS gene of Arabidopsis. Nature 379, 66–69
    OpenUrlCrossRefPubMedWeb of Science
    1. McConnell J.R.,
    2. Barton M.K.
    (1995) Effect of mutations in the PINHEAD gene of Arabidopsis on the formation of shoot apical meristems. Dev.Genet 16, 358–366
    OpenUrlCrossRefWeb of Science
    1. Moussian B.,
    2. Schoof H.,
    3. Haecker A.,
    4. Jurgens G.,
    5. Laux T.
    (1998) Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis. EMBOJ 17, 1799–1809
    OpenUrlCrossRefPubMedWeb of Science
    1. Osterhout J.J.,
    2. Lax S.R.,
    3. Ravel J.M.
    (1983) Factors from wheat germ that enhance the activity of eukaryotic initiation factor eIF-2. J.Biol. Chem 258, 8285–8289
    OpenUrlAbstract/FREE Full Text
    1. Seal S.N.,
    2. Schmidt A.,
    3. Marcus A.
    (1983) Wheat germ eIF2 and CoeIF2. J.Biol.Chem 258, 10573–10576
    OpenUrlAbstract/FREE Full Text
    1. Sinha N.,
    2. Williams R.E.,
    3. Hake S.
    (1993) Overexpression of the maize homeobox gene, Knotted-1, causes a switch from determinate to indeterminate cell fates. GenesDev 7, 878–795
    OpenUrl
    1. Tsay Y.F.,
    2. Frank M.J.,
    3. Page T.,
    4. Dean C.,
    5. Crawford N.M.
    (1993) Identification of a mobile endogenous transposon in Arabidopsisthaliana. Science 260, 342–344
    OpenUrlAbstract/FREE Full Text
    1. Zou C.,
    2. Zhang Z.,
    3. Wu S.,
    4. Osterman J.C.
    (1998) Molecular cloning and characterization of a rabbit eIF2C protein. Gene, 211, 187–194
    OpenUrlCrossRefPubMedWeb of Science
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JOURNAL ARTICLES
The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
K. Lynn, A. Fernandez, M. Aida, J. Sedbrook, M. Tasaka, P. Masson, M.K. Barton
Development 1999 126: 469-481;
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