Advertisement
JOURNAL ARTICLES
A series of novel mutants of Arabidopsis thaliana that are defective in the formation of continuous vascular network: calling the auxin signal flow canalization hypothesis into question
K. Koizumi, M. Sugiyama, H. Fukuda
Development 2000 127: 3197-3204;

Summary

For the genetic analysis of molecular mechanisms underlying temporal and spatial regulation of vascular pattern formation, we isolated mutants of Arabidopsis thaliana that are impaired in vascular patterning. Microscopic examination of the cotyledonary venation of 3,400 M(3) lines led to the identification of 12 mutant lines. Genetic analysis of 8 of these mutant lines indicated that vein pattern formation in these lines resulted from monogenic recessive mutations in 7 different genes, designated VAN1 through VAN7. Mutations in VAN1 through VAN6 genes caused fragmentation (disconnection or partial loss) of lateral veins of the cotyledon and tertiary veins of the rosette leaf whereas they were less injurious to the formation of major veins. Detailed characterization of the van3 mutant using pAthb8::GUS and pTED3::GUS, as molecular markers for the early stage of vascular tissue formation showed that the provascular tissue of the cotyledonary lateral veins was differentiated in fragments during late embryogenesis. These phenotypes of the van mutants are discussed in relation to the auxin signal flow canalization hypothesis and the diffusion-reaction prepattern hypothesis, with the fragility of the continuity in the minor vein formation favoring the latter hypothesis.

REFERENCES

    1. Asai R.,
    2. Taguchi E.,
    3. Kume Y.,
    4. Saito M.,
    5. Kondo S.
    (1999) Zebrafish Leopard gene as a component of the putative reaction-diffusion system. Mech. Dev 89, 87–92
    OpenUrlCrossRefPubMedWeb of Science
    1. Baima S.,
    2. Nobili F.,
    3. Sessa G.,
    4. Lucchetti S.,
    5. Ruberti I.,
    6. Morelli G.
    (1995) The expression of the Athb-8 homeobox gene is restricted to provascualr cells in Arabidopsis thaliana. Development 121, 4171–4182
    OpenUrlAbstract
    1. Berleth T.,
    2. Jurgens G.
    (1993) The role of the monopteros gene in organising the basal body region of the Arabidopsis embryo. Development 118, 575–587
    OpenUrlAbstract
    1. Busch M.,
    2. Mayer U.,
    3. Jurgens G.
    (1996) Molecular analysis of the Arabidopsis pattern formation gene GNOM: gene structure and intragenic complementation. Mol. Gen. Genet 250, 681–691
    OpenUrlPubMedWeb of Science
    1. Carland F. M.,
    2. McHale N. A.
    (1996) LOP1: a gene involved in auxin transport and vascular patterning in Arabidopsis. Development 122, 1811–1819
    OpenUrlAbstract
    1. Carland F. M.,
    2. Berg B. L.,
    3. FitzGerald J. N.,
    4. Jinamornphongs S.,
    5. Nelson T.,
    6. Keith B.
    (1999) Genetic regulation of vascular tissue patterning in arabidopsis. Plant Cell 11, 2123–2137
    OpenUrlAbstract/FREE Full Text
    1. Igarashi M.,
    2. Demura T.,
    3. Fukuda H.
    (1998) Expression of the Zinnia TED3 promoter in developing tracheary elements of transgenic Arabidopsis. Plant Mol. Biol 36, 917–927
    OpenUrlCrossRefPubMedWeb of Science
    1. Jurgens G.,
    2. Mayer U.,
    3. Torres Ruiz R. A.,
    4. Berleth T.,
    5. Misera S.
    (1991) Genetic analysis of pattern formation in the Arabidopsis embryo. Development 1, 27–38
    1. Mattsson J.,
    2. Sung Z. R.,
    3. Berleth T.
    (1999) Responses of plant vascular systems to auxin transport inhibition. Development 126, 2979–2991
    OpenUrlAbstract
    1. Mayer U.,
    2. Buttner G.,
    3. Jurgens G.
    (1993) Apical-basal pattern formation in the Arabidopsis embryo: studies on the role of the gnom gene. Development 117, 149–162
    OpenUrlAbstract
    1. Mayer U.,
    2. Torres Ruiz R. A.,
    3. Berleth T.,
    4. Misera S.,
    5. Jurgens G.
    (1991) Mutations affecting body organization in the Arabidopsis embryo. Nature 353, 402–407
    OpenUrlCrossRef
    1. Meinhardt H.
    (1995) Dynamics of stripe formation. Nature 376, 722–723
    OpenUrlCrossRef
    1. Nelson T.,
    2. Dengler N.
    (1997) Leaf vascular pattern formation. Plant Cell 9, 1121–1135
    OpenUrlFREE Full Text
    1. Przemeck G. K. H.,
    2. Mattsson J.,
    3. Hardtke C. S.,
    4. Sung Z. R.,
    5. Berleth T.
    (1996) Studies on the role of the Arabidopsis gene MONOPTEROS in vascular development and plant cell axialization. Planta 200, 229–237
    OpenUrlPubMedWeb of Science
    1. Sachs T.
    (1968) The role of the root in the induction of xylem differentiation in peas. Ann. Bot 32, 391–399
    OpenUrlAbstract/FREE Full Text
    1. Sachs T.
    (1969) Polarity and the induction of organized vascular tissues. Ann. Bot 33, 263–275
    OpenUrlAbstract/FREE Full Text
    1. Sachs T.
    (1975) The control of the differentiation of vascular networks. Ann. Bot 39, 197–204
    OpenUrlAbstract/FREE Full Text
    1. Sachs T.
    (1991) Cell polarity and tissue patterning in plants. Development 1, 83–93
    1. Shevell D. E.,
    2. Leu W. M.,
    3. Gillmor C. S.,
    4. Xia G.,
    5. Feldmann K. A.,
    6. Chua N. H.
    (1994) EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7. Cell 77, 1051–1062
    OpenUrlCrossRefPubMedWeb of Science
    1. Steinmann T.,
    2. Geldner N.,
    3. Grebe M.,
    4. Mangold S.,
    5. Jackson C. L.,
    6. Paris S.,
    7. Gälweiler L.,
    8. Palme K.,
    9. Jurgens G.
    (1999) Coordinated polar localization of auxin efflux carrier PIN1 by GNOM ARF GEF. Science 286, 316–318
    OpenUrlAbstract/FREE Full Text
    1. Torres Ruiz R. A.,
    2. Jurgens G.
    (1994) Mutations in the FASS gene uncouple pattern formation and morphogenesis in Arabidopsis development. Development 120, 2967–2978
    OpenUrlAbstract
    1. Valvekens D.,
    2. Van Montagu M.,
    3. Van Lijsebettens M.
    (1988) Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc. Natl. Acad. Sci. USA 85, 5536–5540
    OpenUrlAbstract/FREE Full Text
Back to top

 Download PDF

Email
JOURNAL ARTICLES
A series of novel mutants of Arabidopsis thaliana that are defective in the formation of continuous vascular network: calling the auxin signal flow canalization hypothesis into question
K. Koizumi, M. Sugiyama, H. Fukuda
Development 2000 127: 3197-3204;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Alerts

Article navigation

Other journals from The Company of Biologists

Advertisement

Kathryn Virginia Anderson (1952-2020)

Developmental geneticist Kathryn Anderson passed away at home on 30 November 2020. Tamara Caspary, a former postdoc and friend, remembers Kathryn and her remarkable contribution to developmental biology.

Zooming into 2021

In a new Editorial, Editor-in-Chief James Briscoe and Executive Editor Katherine Brown reflect on the triumphs and tribulations of the last 12 months, and look towards a hopefully calmer and more predictable year.

Read & Publish participation extends worldwide

Over 60 institutions in 12 countries are now participating in our Read & Publish initiative. Here, James Briscoe explains what this means for his institution, The Francis Crick Institute. Find out more and view our full list of participating institutions.

Upcoming special issues

Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021

The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022

Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.

Development presents...

Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Sign up to join our next session:

10 February
Time: 13:00 (GMT)
Chaired by: preLights