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JOURNAL ARTICLES
Auxin and ETTIN in Arabidopsis gynoecium morphogenesis
J.L. Nemhauser, L.J. Feldman, P.C. Zambryski
Development 2000 127: 3877-3888;

Summary

The phytohormone auxin has wide-ranging effects on growth and development. Genetic and physiological approaches implicate auxin flux in determination of floral organ number and patterning. This study uses a novel technique of transiently applying a polar auxin transport inhibitor, N-1-naphthylphthalamic acid (NPA), to developing Arabidopsis flowers to further characterize the role of auxin in organogenesis. NPA has marked effects on floral organ number as well as on regional specification in wild-type gynoecia, as defined by morphological and histological landmarks for regional boundaries, as well as tissue-specific reporter lines. NPA's effects on gynoecium patterning mimic the phenotype of mutations in ETTIN, a member of the auxin response factor family of transcription factors. In addition, application of different concentrations of NPA reveal an increased sensitivity of weak ettin alleles to disruptions in polar auxin transport. In contrast, the defects found in spatula gynoecia are partially rescued by treatment with NPA. A model is proposed suggesting an apical-basal gradient of auxin during gynoecium development. This model provides a mechanism linking ETTIN's putative transcriptional regulation of auxin-responsive genes to the establishment or elaboration of tissue patterning during gynoecial development.

REFERENCES

    1. Alvarez J.,
    2. Smyth D. R.
    (1999) CRABS CLAW and SPATULA, two Arabidopsis genes that control carpel development in parallel with AGAMOUS. Development 126, 2377–2386
    OpenUrlAbstract
    1. Bennett S. R. M.,
    2. Alvarez J.,
    3. Bossinger G.,
    4. Smyth D. R.
    (1995) Morphogenesis in pinoid mutants of Arabidopsis thaliana. Plant J 8, 505–520
    1. Bowman J. L.,
    2. Smyth D. R.
    (1999) CRABS CLAW, a gene that regulates carpel and nectary development in Arabidopsis, encodes a novel protein with zinc finger and helix-loop-helix domains. Development 126, 2387–2396
    OpenUrlAbstract
    1. Chen R.,
    2. Hilson P.,
    3. Sedbrook J.,
    4. Rosen E.,
    5. Caspar T.,
    6. Masson P. H.
    (1998) The arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar-auxin-transport efflux carrier. Proc Natl Acad Sci USA 95, 15112–15117
    OpenUrlAbstract/FREE Full Text
    1. Christensen S. K.,
    2. Dagenais N.,
    3. Chory J.,
    4. Weigel D.
    (2000) Regulation of auxin response by the protein kinase PINOID. Cell 100, 469–478
    OpenUrlCrossRefPubMedWeb of Science
    1. Galweiler L.,
    2. Guan C.,
    3. A M.,
    4. Wisman E.,
    5. Mendgen K.,
    6. Yephremov A.,
    7. Palme K.
    (1998) Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue. Science 282, 2226–2230
    OpenUrlAbstract/FREE Full Text
    1. Hardtke C. S.,
    2. Berleth T.
    (1998) The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. EMBO 17, 1405–1411
    OpenUrlAbstract
    1. Kim J.,
    2. Harter K.,
    3. Theologis A.
    (1997) Protein-protein interactions among the Aux-IAA proteins. Proc. Natl. Acad. Sci. USA 94, 11786–11791
    OpenUrlAbstract/FREE Full Text
    1. Luschnig C.,
    2. Gaxiola R. A.,
    3. Grisafi P.,
    4. Fink G. R.
    (1998) EIR1, a root-specific protein involved in auxin transport, is required for gravitropism in Arabidopsis thaliana. Genes Dev 12, 2175–2187
    OpenUrlAbstract/FREE Full Text
    1. Mattsson J.,
    2. Sung Z. R.,
    3. Berleth T.
    (1999) Responses of plant vascular systems to auxin transport inhibition. Development 126, 2979–2991
    OpenUrlAbstract
    1. Muller A.,
    2. Guan C.,
    3. Galweiler L.,
    4. Tanzler P.,
    5. Huijser P.,
    6. Marchant A.,
    7. Parry G.,
    8. Bennett M.,
    9. Wisman E.,
    10. Palme K.
    (1998) AtPIN2 defines a locus of Arabidopsis for root gravitropism control. EMBO J 17, 6903–6911
    OpenUrlAbstract
    1. Oka M.,
    2. Miyamoto K.,
    3. Okada K.,
    4. Ueda J.
    (1999) Auxin polar transport and flower formation in Arabidopsis thaliana transformed with indoleacetamide hydrolase (iaaH) gene. Plant Cell Physiol 40, 231–237
    OpenUrlAbstract/FREE Full Text
    1. Okada K.,
    2. Ueda J.,
    3. Komaki M. K.,
    4. Bell C. J.,
    5. Shimura Y.
    (1991) Requirement of the auxin polar transport system in early stages of Arabidopsis floral bud formation. Plant Cell 3, 677–684
    OpenUrlAbstract/FREE Full Text
    1. Przemeck G. K. H.,
    2. Mattsson J.,
    3. Hardtke C. S.,
    4. Sung Z. R.,
    5. Berleth T.
    (1996) Studies on the role of the Arabidopsis gene MONOPTEROS in vascular development and plant cell axialization. Planta 200, 229–237
    OpenUrlPubMedWeb of Science
    1. Reed R. C.,
    2. Brady S. R.,
    3. Muday G. K.
    (1998) Inhibition of auxin movement from the shoot into the root inhibits lateral root development in Arabidopsis. Plant Physiol 118, 1369–1378
    OpenUrlAbstract/FREE Full Text
    1. Reinhardt D.,
    2. Mandel T.,
    3. Kuhlemeier C.
    (2000) Auxin Regulates the Initiation and Radial Position of Plant Lateral Organs. Plant Cell 12, 507–518
    OpenUrlAbstract/FREE Full Text
    1. Roe J. L.,
    2. Nemhauser J. L.,
    3. Zambryski P. C.
    (1997) TOUSLED participates in apical tissue formation during gynoecium development in Arabidopsis. Plant Cell 9, 335–353
    OpenUrlAbstract/FREE Full Text
    1. Ruegger M.,
    2. Dewey E.,
    3. Hobbie L.,
    4. Brown D.,
    5. Bernasconi P.,
    6. Turner J.,
    7. Muday G.,
    8. Estelle M.
    (1997) Reduced naphthylphthalamic acid binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport and diverse morphological defects. Plant Cell 9, 745–757
    OpenUrlAbstract/FREE Full Text
    1. Sabatini S.,
    2. Beis D.,
    3. Wolkenfelt H.,
    4. Murfett J.,
    5. Guilfoyle T.,
    6. Malamy J.,
    7. Benfey P.,
    8. Leyser O.,
    9. Bechtold N.,
    10. Weisbeek P.,
    11. et al.
    (1999) An auxin-dependent distal organizer of pattern and polarity in the Arabidopsis root. Cell 99, 463–472
    OpenUrlCrossRefPubMedWeb of Science
    1. Sessions A.,
    2. Nemhauser J. L.,
    3. McColl A.,
    4. Roe J. L.,
    5. Feldmann K. A.,
    6. Zambryski P. C.
    (1997) ETTIN patterns the Arabidopsis floral meristem and reproductive organs. Development 124, 4481–4491
    OpenUrlAbstract
    1. Sessions R. A.
    (1997) Arabidopsis (Brassicaceae) flower development and gynoecium patterning in wild type and ettin mutants. Am. J. Bot 84, 1179–1191
    OpenUrlAbstract/FREE Full Text
    1. Sessions R. A.,
    2. Zambryski P. C.
    (1995) Arabidopsis gynoecium structure in the wild type and in ettin mutants. Development 121, 1519–1532
    OpenUrlAbstract
    1. Smyth D. R.,
    2. Bowman J. L.,
    3. Meyerowitz E. M.
    (1990) Early flower development in Arabidopsis. Plant Cell 2, 755–767
    OpenUrlAbstract/FREE Full Text
    1. Steinmann T.,
    2. Geldner N.,
    3. Grebe M.,
    4. Mangold S.,
    5. Jackson C. L.,
    6. Paris S.,
    7. Galweiler L.,
    8. Palme K.,
    9. Jurgens G.
    (1999) Coordinated polar localization of auxin efflux carrier PIN1 by GNOM ARF GEF. Science 286, 316–318
    OpenUrlAbstract/FREE Full Text
    1. Sundaresan V.,
    2. Springer P.,
    3. Volpe T.,
    4. Haward S.,
    5. Jones J. D.,
    6. Dean C.,
    7. Ma H.,
    8. Martienssen R.
    (1995) Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes Dev 9, 1797–1810
    OpenUrlAbstract/FREE Full Text
    1. Thomson K.,
    2. Hertel R.,
    3. Muller S.,
    4. Tavares J.
    (1973) 1-N-Naphthylphthalamic Acid and 2,3,5-Triiodobenzoic Acid: in vitro binding to particulate cell fractions and action on auxin transport in corn coleoptiles. Planta 109, 337–352
    OpenUrlCrossRefPubMedWeb of Science
    1. Toriyama K.,
    2. Thorsness M. K.,
    3. Nasrallah J. B.,
    4. Nasrallah M. E.
    (1991) A Brassica S locus gene promoter directs sporophytic expression in the anther tapetum of transgenic Arabidopsis. Dev. Biol 143, 427–431
    OpenUrlCrossRefPubMedWeb of Science
    1. Tuominen H.,
    2. Puech L.,
    3. Fink S.,
    4. Sundberg B.
    (1997) A radial concentration gradient of indole-3-acetic acid is related to secondary xylem development in hybrid aspen. Plant Physiol 115, 577–585
    OpenUrlAbstract
    1. Uggla C.,
    2. Mellerowicz E. J.,
    3. Sundberg B.
    (1998) Indole-3-acetic acid controls cambial growth in scots pine by positional signaling. Plant Physiol 117, 113–121
    OpenUrlAbstract/FREE Full Text
    1. Uggla C.,
    2. Moritz T.,
    3. Sandberg G.,
    4. Sundberg B.
    (1996) Auxin as a positional signal in pattern formation in plants. Proc. Natl. Acad. Sci. USA 93, 9282–9286
    OpenUrlAbstract/FREE Full Text
    1. Ulmasov T.,
    2. Hagen G.,
    3. Guilfoyle T. J.
    (1997) ARF1, a transcription factor that binds to auxin response elements. Science 276, 1865–1868
    OpenUrlAbstract/FREE Full Text
    1. Ulmasov T.,
    2. Hagen G.,
    3. Guilfoyle T. J.
    (1999) Activation and repression of transcription by auxin-response factors. Proc. Natl. Acad. Sci. USA 96, 5844–5849
    OpenUrlAbstract/FREE Full Text
    1. Ulmasov T.,
    2. Hagen G.,
    3. Guilfoyle T. J.
    (1999) Dimerization and DNA binding of auxin response factors. Plant J 19, 309–319
    OpenUrlCrossRefPubMedWeb of Science
    1. Ulmasov T.,
    2. Murfett J.,
    3. Hagen G.,
    4. Guilfoyle T. J.
    (1997) Aux-IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell 9, 1963–1971
    OpenUrlAbstract/FREE Full Text
    1. Utsuno K.,
    2. Shikanai T.,
    3. Yamada Y.,
    4. Hashimoto T.
    (1998) Agr, an Agravitropic locus of Arabidopsis thaliana, encodes a novel membrane-protein family member. Plant Cell Physiol 39, 1111–1118
    OpenUrlAbstract/FREE Full Text
    1. Vivian-Smith A.,
    2. Koltunow A. M.
    (1999) Genetic analysis of growth-regulator-induced parthenocarpy in Arabidopsis. Plant Physiol 121, 437–451
    OpenUrlAbstract/FREE Full Text
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JOURNAL ARTICLES
Auxin and ETTIN in Arabidopsis gynoecium morphogenesis
J.L. Nemhauser, L.J. Feldman, P.C. Zambryski
Development 2000 127: 3877-3888;
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