DEV02401 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Comparison of protein levels after overexpression of Ft and Ds constructs in the posteriors of wild-type discs using en-gal4. Discs expressing full length and truncated Ft constructs were stained with antiserum against the C terminus of Ft, while discs expressing full-length and truncated Ds constructs were stained with raised against both the N terminus (Strutt and Strutt., 2002) and C terminus (Yang et al., 2002) of Ds. In order to compare levels, identical confocal settings were used. Expression was largely equivalent between all the Ft and all the Ds constructs, and well above the levels of the endogenous proteins.

• Supplemental Figure 2 -

  Fig. S2. More comparisons of overgrowth in wing and eye antennal imaginal discs. Typical wing discs (A-G) ad eye-antennal discs (H-N)for each genotype are shown at the same magnification. ft^� is ft^G-rv/ft^fd, ds ^� is ds ^UAO71, and ds ^� ft^� is ds ^UAO71 ft^G-rv/ds ^UAO71 ft^fd. (A,H) Wild type. (B,I) Overgrowth in ft^�. (C,J) Rescue of ft^� overgrowth with UAS-ft and da-gal4. (D,K) Rescue of ft^�overgrowth with UAS-ftΔECD and da-gal4. (E,L) Potentiation of ft^�overgrowth with UAS-ftΔICD and da-gal4. (F,M) Mild overgrowth in ds^�. (G,N) Potentiation of ft^� overgrowth by ds^�.

• Supplemental Figure 3 -

  Fig. S3. ft^fd clones (lacking GFP, green) in everted wing discs 5 hours after pupariation. Using this stage allows the easy examination of proximal wing blade tissues normally hidden in a fold at late third instar. Wing blade cells at this stage show the same sensitivity to gains in Wg signaling as at late third instar (Blair, 1994). (A,B) Anti-Dll staining in a wing containing smaller clones generated at late second-early third instar. The normal distal-to-proximal gradient of Dll is visible. None of the clones showed elevated levels of Dll when compared with their wild-type neighbors (high magnification image in B). (C,D) Anti-Vg staining in a wing containing larger clones generated at late first-early second instar. The normal distal-to-proximal gradient of Vg is visible. Although egions of some extremely overgrown clones showed distortions of the Vg staining pattern, many regions of overgrowth showed the same levels of staining as the neighboring wild-type cells (arrows). A large distal clone, clearly overgrown in comparison to its GFP/GFP twin spot, showed no change in Vg levels (high magnification image in D).

• Supplemental Figure 4 -

  Fig. S4. Hair polarity in adult wings misexpressing full-length or deleted ft constructs. Either the proximal region near the convergence of L2 and L3 (A,C,E,G,I,K) or the distal region between L3 and L4 (B,D,F,H,J,L) is shown. (A,B) Wild type. (C-H) Proximal but not distal PCP defects were induced in wild-type wings with act-gal4 and UAS-ft (C,D), UAS-ftΔECD (E,F) or UAS-ftΔICD (G,H). Defects were milder with UAS-ftΔECD. (I-L) Apparent rescue of PCP defects in distal but not proximal regions of ft^G-rv/ft^fd wings with act-gal4 and UAS-ft (I,J) or UAS-ftΔECD (K.L).

• Supplemental Figure 5 -

  Fig. S5. Hair polarity in adult wings misexpressing full-length or deleted ds constructs using ds-gal4. (A,B) The expression pattern of ds-gal4 in an early pupal wing, monitored by UAS-GFP (green in A, white in B) and anti-DSRF staining of intervein tissue (purple in A). The expression is similar to the anti-Ds staining pattern at this stage (Matakatsu and Blair, 2004). (C-F) PCP defects in proximal wings. (C) PCP disruption in ds⁰⁵¹⁴²/ds^gal4. (D,E) Partial rescue of PCP defects by UAS-ds (D) or UAS-dsΔICD (E). (F) UAS-dsΔECD did not rescue PCP.