DEV003798 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Oct4, Cdx2 and Nanog expression. (A) Oct4, (B) Cdx2 and (C) Nanog in freshly collected embryos at 70, 90 and 115 hp-hCG. White and green arrowheads indicate nuclei of interest in inside and outside cells, respectively. Actin (red) marks the cell membranes. All images are single optical sections. Scale bar: 20 μm.

• Supplemental Figure S2 -

  Fig. S2. Correct patterning depends on cell cycle progression. (A) Development of embryos either treated with E-cadherin inhibitory antibody DECMA-1 (red bar) and washed, or left untreated.bI, bII and bIII, blastocyst stages I, II and III respectively. White asterisks mark embryos at blastocyst II stage. (B) Cdx2 and Nanog expression in DECMA-1-treated and untreated embryos at 80 hp-hCG (red asterisks in A and B). DNA staining provides a reference value. Actin (red) marks the cell membranes. All images are single optical sections. See main text for a detailed description of the experiments. Scale bars: 100 μm in A; 20 μm in B.

• Supplemental Movie S1 -

  Movie 1. Cdx2 expression is variable, independent of Oct4. Actin (red) marks the cell membranes. This embryo is the same one as in Fig. 4A, at 20 hp-c. Each frame is one single optical section of 1 μm. Scale bar: 20 μm.

• Supplemental Movie S2 -

  Movie 2. The expression of Cdx2 and Nanog is variable and they are mutually independent. Actin (red) marks the cell membranes. This embryo is the same one as in Fig. 3B, at 20 hp-c. Each frame is one single optical section of 1 μm. Scale bar: 20 μm.

• Supplemental Movie S3 -

  Movie 3. Blastocyst formation during normal development and after delayed compaction as a result of DECMA-1 treatment. This time-lapse movie corresponds to the series in Fig. S2A rotated by 180°. Time in HH:MM. Scale bar: 100 μm.