DEV016253 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The expression level of Lgl and pLgl in the wild type, Lgl- and Lgl-3A-overexpressing ovaries. (A) Western blot analyses with the anti-Lgl, anti-pLgl and anti-α-Tubulin antibodies (loading control), indicating the levels of Lgl and pLgl in the wild-type (mat-Gal4 driver only), Lgl-overexpressing and Lgl-3A-overexpressing ovaries. (B) Comparison of the relative levels of pLgl and Lgl in the wild-type, Lgl-overexpressing and Lgl-3A-overexpressing ovaries. Values were means of the three independent experiments, which were normalized with the amounts of α-Tubulin. The Lgl and pLgl levels in the wild type were set to 1.

• Supplemental Figure S2 -

  Fig. S2. Stau was partially mislocalized in stage 9 egg chambers with Lgl overexpression. In stage 9 egg chambers with Lgl-3A overexpression, Stau was frequently mislocalized to the center of the oocyte.

• Supplemental Figure S3 -

  Fig. S3. bcd mRNA in wild-type egg chambers. There is disc-shaped localization in the oocyte anterior (A), but in Lgl-3A overexpression bcd sometimes localized at the posterior of the oocyte.

• Supplemental Figure S4 -

  Fig. S4. Lgl localization. Lgl was localized at the cortex when Lgl-3A was co-overexpressed with aPKC^ΔN-GFP in the egg chambers with the driver Mat-Gal4.