A mutation in the flt4 kinase domain is responsible for the expando phenotype. (A) Meiotic mapping links the expando locus to a region of chromosome 14 containing flt4. Analysis of 131 zebrafish embryos (262 meioses) identifies two recombination events at the marker z43267 and three independent recombinants at the marker z26376, thereby localising the mutation to a region containing flt4. (B,C) Sequencing of the coding exons of flt4 reveals a T>G mutation (see chromatogram in B) that changes a highly conserved isoleucine to a serine at position 1042 of the predicted zebrafish Flt4 protein sequence. Flt4 receptor structure is depicted, with Ig domain (I-VII), transmembrane domain (TM) and split tyrosine kinase domain (TK1 and TK2). Alignment of conserved sequences (C) demonstrates that I1042 is a conserved residue, with grey shading indicating non-conserved residues. (D) Tyrosine phosphorylation (pY; upper panel, phosphotyrosine blot) was seen upon in vitro stimulation with VEGFC of wild-type human FLT4, but not of I1034S mutant FLT4. Lower panel (Flt4 protein blot) indicates the presence of FLT4 protein post-transfection in the assayed samples.

Zebrafish flt4^hu4602 mutants lack the PHBC defects observed upon flt4 MO, vegfc MO or sFLT4 mRNA injection. (A-E) Angiogenesis of the primordial hindbrain channel (PHBC) fails to occur in flt4 MO-injected (5 ng/embryo) (C), sFLT4 mRNA-injected (200 pg/embryo) (D) and vegfc MO-injected (E) embryos (yellow asterisk) as compared with wild-type controls (A), but is unaffected (yellow arrow) in individually genotyped flt4^hu4602 mutants (B) at 28 hpf. (F,G) Angiogenesis of the PHBC and lymphangiogenesis respond differently to injection of flt4 MO, vegfc MO and sFLT4 mRNA. Injection of flt4 MO (5 ng/embryo) inhibits PHBC development in 29% of embryos at 28 hpf but inhibits lymphangiogenesis [thoracic duct (TD) scored as readout] with 100% efficiency at 4 dpf in the same injection (n=55 embryos scored). vegfc MO (5 ng/embryo) inhibits PHBC development in 22% of embryos (n=41) and TD development in 97% of embryos in the same injection (n=37). sFLT4 mRNA (200 pg/embryo) inhibits PHBC development in 42% of embryos and TD development in 100% of embryos in the same injection (n=120). Uninjected control embryos showed normal PHBC and TD development in 100% of cases (n=20) at 28 hpf and 4 dpf (F,G).

The dll4 morphant hyperbranching phenotype is rescued in flt4^hu4602 mutants, vegfc morphants and sFLT4 mRNA-injected embryos. (A-D) Lateral views of uninjected control (A) and flt4^hu4602 mutant (B) as compared with wild-type (C) and flt4^hu4602 mutant (D) zebrafish embryos injected with dll4 MO. Images were taken in the TG(flt1:YFP)^hu4624 background, labelling trunk arteries to reveal the failure to form intersegmental venous sprouts in expando mutants (B, yellow arrows). The dll4 morphant arterial hyperbranching phenotype (C) is rescued in flt4^hu4602 mutants (D). Venous sprouts were identified by the absence of flt1:YFP expression in ISVs (brackets). Embryos at 72 hpf. (E) Arterial hyperbranching phenotypes were scored post-injection of dll4 MO and subsequently genotyped. The mutant genotype was almost completely absent from the severe category (e.g. C) (1/29 embryos, *P<0.01 by χ² test) and was enriched in the category not displaying hyperbranching (e.g. D) (21/55 embryos, ^#P<0.01 by χ² test). (F) In transgenic TG(flt1:YFP)^hu4624 embryos, co-injection with dll4 MO together with vegfc MO (green bars, n=57 embryos), or of dll4 MO together with sFLT4 mRNA (200 pg/embryo) (red bars, n=67 embryos), led to rescue of the arterial hyperbranching phenotype observed upon injection of dll4 MO alone (blue bars, n=81 embryos). Arterial hyperbranching phenotypes were categorised as no hyperbranching (none), mild or severe (see Fig. S1 in the supplementary material).

Sprouting intersegmental arteries express flt4 and are sensitised to exogenously introduced Vegfc in the absence of dll4. (A-E) At 24 hpf, the expression of flt4 is present in all arterial cells (A) and is unaltered upon loss of dll4 (B) (n=40 uninjected control and n=40 dll4 MO-injected embryos scored). By 26 hpf (C), flt4 is downregulated in the dorsal aorta (n=20 embryos). Analysis of a flt4 promoter-driven transgenic line identifies YFP expression in all arterial cells at 24 hpf (D). The expression of YFP is unaltered in dll4 MO-injected embryos (E). (F,G) At 24 hpf, the expression of vegfc is present in the dorsal aorta (F) and is unaltered in dll4 MO-injected embryos (G) (n=20 uninjected and n=20 MO-injected embryos). (H,I) The expression of vegfd in the tailbud is unaltered in dll4 MO-injected (I) as compared with uninjected control (H) embryos (n=20 uninjected and n=20 MO-injected embryos). (J,K) The expression of the arterial marker gene hey2 is unaltered in dll4 MO-injected (K) as compared with uninjected control (J) embryos (n=20 uninjected and n=20 MO-injected embryos). (L-R) dll4 morphants are sensitised to exogenously introduced vegfc mRNA during arterial angiogenesis. Forced expression by the injection of mRNA encoding full-length zebrafish Vegfc into dll4 MO-injected embryos (n=133) (P) leads to aberrant ectopic bilateral turning of ISAs by 28 hpf (yellow arrows), which was never observed in wild-type embryos (L) or in those injected with dll4 MO only (n=90) (M). ISAs rarely turn bilaterally upon injection of vegfc mRNA only (n=106) (N), whereas injection of full-length vegfd mRNA robustly showed this phenotype without (n=211) (O) or with (n=227) (Q) dll4 MO. For each of the conditions tested, the percentage of embryos showing wild-type, mild (aberrant ISAs spanning two somites) or severe (aberrant ISAs spanning more than two somites) ISA phenotypes is shown in R.

Overexpression of dll4 reduces sprouting from the PCV. (A,B) Injection of mRNA encoding full-length Dll4 into TG(flt1:YFP)^hu4624 zebrafish embryos leads to a reduction in the number of venous sprouts in injected (B) as compared with uninjected control (A) embryos at 52 hpf. ISAs that connect directly to the dorsal aorta (red bar) are indicated by yellow arrows. Venous spouts (yellow brackets) can be readily identified by the absence of TG(flt1:YFP)^hu4624 expression in the ventral component of an intersegmental vessel. Venous sprouts connect directly to the posterior cardinal vein (blue bar) from which they derive during secondary sprouting. (C) Quantification of the number of venous sprouts (y-axis) on one side of the embryo across 12 segments anterior to the cloaca (n=23 embryos scored for both dll4 MO-injected and uninjected controls). dll4 MO-injected embryos show a significant decrease in the number of intersegmental venous sprouts (P<0.001, Mann-Whitney rank sum test).