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Research Article
Molecular dissection of integrin signalling proteins in the control of mammary epithelial development and differentiation
Nasreen Akhtar, Rebecca Marlow, Elise Lambert, Franziska Schatzmann, Emma T. Lowe, Julia Cheung, Elad Katz, Weiping Li, Chuanyue Wu, Shoukat Dedhar, Matthew J. Naylor, Charles H. Streuli
Development 2009 136: 1019-1027; doi: 10.1242/dev.028423
Nasreen Akhtar
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Rebecca Marlow
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Elise Lambert
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Franziska Schatzmann
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Emma T. Lowe
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Julia Cheung
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Elad Katz
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Weiping Li
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Chuanyue Wu
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Shoukat Dedhar
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Matthew J. Naylor
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Charles H. Streuli
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Data supplements

  • DEV028423 Supplementary Material

    Files in this Data Supplement:

    • Supplemental Figure S1 -

      Fig. S1. ILK expression is retained in myoepithelial cells in Ilk−/− mammary glands. (A) Immunohistochemical analysis of Ilkfx/fx;WapiCreTg/• mammary gland wax sections demonstrate ILK-positive brown staining in cells at edges of the alveoli. (B-D) This is also evident by immunofluorescence staining with anti-ILK antibody (red), which colocalises with smooth muscle actin (SMA), a marker for myoepithelial cells (green). Scale bar: 30 µm.

    • Supplemental Figure S2 -

      Fig S2. Ad-Cre infection does not affect either ILK or β-casein expression in wild-type MEC. Wild-type primary mammary epithelial cells were uninfected or infected with adenovirus encoding β-Gal (Ad-LacZ) or Cre (Ad-Cre) and then plated onto BM matrix in growth medium for 3 days. Differentiation was induced by prolactin stimulation 24 hours before harvest. The levels of β-casein and ILK are not affected by viral infection. The faint band below β-gal (red asterisk) is non-specific.

    • Supplemental Figure S3 -

      Fig. S3. PKB and ERK activation in the absence of ILK. (A) Ilkfx/fx primary mammary epithelial cells were infected with Ad-LacZ or Ad-Cre and plated onto BM-matrix in growth medium for 3 days. Differentiation was induced by prolactin stimulation 24 hours before harvest. Cell lysates were analysed by immunoblotting. Ad-Cre infected cells show similar steady-state levels of phosphorylated GSK3β-S9 as control or mock-infected cells. (B,C) Acute stimulation of the PKB and ERK pathways in the absence of ILK was measured by culturing infected cells on BM-matrix either without insulin for 3 days and then treating with insulin for 30 minutes, or by serum starvation for 4 hours before a 30-minute EGF treatment, respectively. (B) In the Ad-Cre-infected cells, PKB is still phosphorylated on both Ser473 and Thr308 after insulin stimulation. (C) Phosphorylation of ERK takes place in response to EGF stimulation in both control cells and those following Ad-Cre infection.

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Research Article
Molecular dissection of integrin signalling proteins in the control of mammary epithelial development and differentiation
Nasreen Akhtar, Rebecca Marlow, Elise Lambert, Franziska Schatzmann, Emma T. Lowe, Julia Cheung, Elad Katz, Weiping Li, Chuanyue Wu, Shoukat Dedhar, Matthew J. Naylor, Charles H. Streuli
Development 2009 136: 1019-1027; doi: 10.1242/dev.028423
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Research Article
Molecular dissection of integrin signalling proteins in the control of mammary epithelial development and differentiation
Nasreen Akhtar, Rebecca Marlow, Elise Lambert, Franziska Schatzmann, Emma T. Lowe, Julia Cheung, Elad Katz, Weiping Li, Chuanyue Wu, Shoukat Dedhar, Matthew J. Naylor, Charles H. Streuli
Development 2009 136: 1019-1027; doi: 10.1242/dev.028423

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An interview with Swathi Arur

Swathi Arur joined the team at Development as an Academic Editor in 2020. Her lab uses multidisciplinary approaches to understand female germline development and fertility. We met with her over Zoom to hear more about her life, her career and her love for C. elegans.


Jim Wells and Hanna Mikkola join our team of Editors

We are pleased to welcome James (Jim) Wells and Hanna Mikkola to our team of Editors. Jim joins us a new Academic Editor, taking over from Gordan Keller, and Hanna joins our team of Associate Editors. Find out more about their research interests and areas of expertise.


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