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RESEARCH ARTICLE
Retinoic acid synthesis and autoregulation mediate zonal patterning of vestibular organs and inner ear morphogenesis
Kazuya Ono, Lisa L. Sandell, Paul A. Trainor, Doris K. Wu
Development 2020 147: dev192070 doi: 10.1242/dev.192070 Published 7 August 2020
Kazuya Ono
1National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA
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  • ORCID record for Kazuya Ono
Lisa L. Sandell
2Department of Oral Immunology and Infectious Diseases, School of Dentistry, University of Louisville, Louisville, KY 40201, USA
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Paul A. Trainor
3Stowers Institute for Medical Research, Kansas City, MO 64110, USA
4Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA
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Doris K. Wu
1National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA
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  • For correspondence: wud@nidcd.nih.gov

Handling Editor: Patrick Tam

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ABSTRACT

Retinoic acid (RA), a vitamin A (retinol) derivative, has pleiotropic functions during embryonic development. The synthesis of RA requires two enzymatic reactions: oxidation of retinol into retinaldehyde by alcohol dehydrogenases (ADHs) or retinol dehydrogenases (RDHs); and oxidation of retinaldehyde into RA by aldehyde dehydrogenases family 1, subfamily A (ALDH1as), such as ALDH1a1, ALDH1a2 and ALDH1a3. Levels of RA in tissues are regulated by spatiotemporal expression patterns of genes encoding RA-synthesizing and -degrading enzymes, such as cytochrome P450 26 (Cyp26 genes). Here, we show that RDH10 is important for both sensory and non-sensory formation of the vestibule of the inner ear. Mice deficient in Rdh10 exhibit failure of utricle-saccule separation, otoconial formation and zonal patterning of vestibular sensory organs. These phenotypes are similar to those of Aldh1a3 knockouts, and the sensory phenotype is complementary to that of Cyp26b1 knockouts. Together, these results demonstrate that RDH10 and ALDH1a3 are the key RA-synthesis enzymes involved in vestibular development. Furthermore, we discovered that RA induces Cyp26b1 expression in the developing vestibular sensory organs, which generates the differential RA signaling required for zonal patterning.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: K.O., D.K.W.; Methodology: K.O., D.K.W.; Formal analysis: K.O.; Investigation: K.O.; Resources: L.L.S., P.A.T.; Data curation: K.O.; Writing - original draft: K.O.; Writing - review & editing: K.O., L.L.S., P.A.T., D.K.W.; Supervision: D.K.W.; Project administration: D.K.W.; Funding acquisition: P.A.T., D.K.W.

  • Funding

    This research was supported by funds from the intramural program at the National Institute on Deafness and Other Communication Disorders (D.K.W.) and by the National Institute for Dental and Craniofacial Research (DE016082 to P.A.T.) and the Stowers Institute for Medical Research (P.A.T.). Deposited in PMC for release after 12 months.

  • Data availability

    All data are available from the corresponding author upon reasonable request.

  • Received April 27, 2020.
  • Accepted July 1, 2020.
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Keywords

  • RDH10
  • CYP26b1
  • ALDH1a3
  • Retinoic acid
  • Striola
  • Patterning

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RESEARCH ARTICLE
Retinoic acid synthesis and autoregulation mediate zonal patterning of vestibular organs and inner ear morphogenesis
Kazuya Ono, Lisa L. Sandell, Paul A. Trainor, Doris K. Wu
Development 2020 147: dev192070 doi: 10.1242/dev.192070 Published 7 August 2020
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RESEARCH ARTICLE
Retinoic acid synthesis and autoregulation mediate zonal patterning of vestibular organs and inner ear morphogenesis
Kazuya Ono, Lisa L. Sandell, Paul A. Trainor, Doris K. Wu
Development 2020 147: dev192070 doi: 10.1242/dev.192070 Published 7 August 2020

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