Handling Editor: François Guillemot
ABSTRACT
Neuronal pruning is essential for proper wiring of the nervous systems in invertebrates and vertebrates. Drosophila ddaC sensory neurons selectively prune their larval dendrites to sculpt the nervous system during early metamorphosis. However, the molecular mechanisms underlying ddaC dendrite pruning remain elusive. Here, we identify an important and cell-autonomous role of the membrane protein Raw in dendrite pruning of ddaC neurons. Raw appears to regulate dendrite pruning via a novel mechanism, which is independent of JNK signaling. Importantly, we show that Raw promotes endocytosis and downregulation of the conserved L1-type cell-adhesion molecule Neuroglian (Nrg) prior to dendrite pruning. Moreover, Raw is required to modulate the secretory pathway by regulating the integrity of secretory organelles and efficient protein secretion. Mechanistically, Raw facilitates Nrg downregulation and dendrite pruning in part through regulation of the secretory pathway. Thus, this study reveals a JNK-independent role of Raw in regulating the secretory pathway and thereby promoting dendrite pruning.
Footnotes
Competing interests
The authors declare no competing or financial interests.
Author contributions
Conceptualization: F.Y.; Methodology: M.R., L.Y.C.; Validation: M.R., F.Y.; Formal analysis: M.R., S.B., L.Y.C., Q.W., F.Y.; Investigation: M.R., S.B., L.Y.C., Q.W., F.Y.; Resources: M.R., S.B., Q.W.; Data curation: M.R.; Writing - original draft: M.R., F.Y.; Writing - review & editing: F.Y.; Visualization: M.R., S.B., L.Y.C., Q.W.; Supervision: F.Y.; Project administration: F.Y.; Funding acquisition: F.Y.
Funding
This work was funded by the Temasek Life Sciences Laboratory Singapore (TLL-2040) and by the National Research Foundation Singapore (SBP-P3 and SBP-P8) (both to F.Y.).
Supplementary information
Supplementary information available online at https://dev.biologists.org/lookup/doi/10.1242/dev.191155.supplemental
- Received April 3, 2020.
- Accepted September 8, 2020.
Log in using your username and password
Log in through your institution
Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$30.00 .
Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.
Article navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Other journals from The Company of Biologists
Kathryn Virginia Anderson (1952-2020)
Developmental geneticist Kathryn Anderson passed away at home on 30 November 2020. Tamara Caspary, a former postdoc and friend, remembers Kathryn and her remarkable contribution to developmental biology.
Zooming into 2021
In a new Editorial, Editor-in-Chief James Briscoe and Executive Editor Katherine Brown reflect on the triumphs and tribulations of the last 12 months, and look towards a hopefully calmer and more predictable year.
Read & Publish participation extends worldwide
Over 60 institutions in 12 countries are now participating in our Read & Publish initiative. Here, James Briscoe explains what this means for his institution, The Francis Crick Institute. Find out more and view our full list of participating institutions.
Upcoming special issues
Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021
The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022
Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.
Development presents...
Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Sign up to join our next session:
10 February
Time: 13:00 (GMT)
Chaired by: preLights