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RESEARCH ARTICLE
The membrane protein Raw regulates dendrite pruning via the secretory pathway
Menglong Rui, Shufeng Bu, Liang Yuh Chew, Qiwei Wang, Fengwei Yu
Development 2020 147: dev191155 doi: 10.1242/dev.191155 Published 9 October 2020
Menglong Rui
1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
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Shufeng Bu
1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
2Department of Biological Sciences, National University of Singapore, Singapore 117543
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Liang Yuh Chew
1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
2Department of Biological Sciences, National University of Singapore, Singapore 117543
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Qiwei Wang
1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
2Department of Biological Sciences, National University of Singapore, Singapore 117543
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Fengwei Yu
1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604
2Department of Biological Sciences, National University of Singapore, Singapore 117543
3NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences, Singapore 117456
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  • ORCID record for Fengwei Yu
  • For correspondence: fengwei@tll.org.sg

Handling Editor: François Guillemot

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ABSTRACT

Neuronal pruning is essential for proper wiring of the nervous systems in invertebrates and vertebrates. Drosophila ddaC sensory neurons selectively prune their larval dendrites to sculpt the nervous system during early metamorphosis. However, the molecular mechanisms underlying ddaC dendrite pruning remain elusive. Here, we identify an important and cell-autonomous role of the membrane protein Raw in dendrite pruning of ddaC neurons. Raw appears to regulate dendrite pruning via a novel mechanism, which is independent of JNK signaling. Importantly, we show that Raw promotes endocytosis and downregulation of the conserved L1-type cell-adhesion molecule Neuroglian (Nrg) prior to dendrite pruning. Moreover, Raw is required to modulate the secretory pathway by regulating the integrity of secretory organelles and efficient protein secretion. Mechanistically, Raw facilitates Nrg downregulation and dendrite pruning in part through regulation of the secretory pathway. Thus, this study reveals a JNK-independent role of Raw in regulating the secretory pathway and thereby promoting dendrite pruning.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: F.Y.; Methodology: M.R., L.Y.C.; Validation: M.R., F.Y.; Formal analysis: M.R., S.B., L.Y.C., Q.W., F.Y.; Investigation: M.R., S.B., L.Y.C., Q.W., F.Y.; Resources: M.R., S.B., Q.W.; Data curation: M.R.; Writing - original draft: M.R., F.Y.; Writing - review & editing: F.Y.; Visualization: M.R., S.B., L.Y.C., Q.W.; Supervision: F.Y.; Project administration: F.Y.; Funding acquisition: F.Y.

  • Funding

    This work was funded by the Temasek Life Sciences Laboratory Singapore (TLL-2040) and by the National Research Foundation Singapore (SBP-P3 and SBP-P8) (both to F.Y.).

  • Supplementary information

    Supplementary information available online at https://dev.biologists.org/lookup/doi/10.1242/dev.191155.supplemental

  • Received April 3, 2020.
  • Accepted September 8, 2020.
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Keywords

  • Pruning
  • Raw
  • JNK signaling
  • Neuroglian
  • Axon
  • Dendrite

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RESEARCH ARTICLE
The membrane protein Raw regulates dendrite pruning via the secretory pathway
Menglong Rui, Shufeng Bu, Liang Yuh Chew, Qiwei Wang, Fengwei Yu
Development 2020 147: dev191155 doi: 10.1242/dev.191155 Published 9 October 2020
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RESEARCH ARTICLE
The membrane protein Raw regulates dendrite pruning via the secretory pathway
Menglong Rui, Shufeng Bu, Liang Yuh Chew, Qiwei Wang, Fengwei Yu
Development 2020 147: dev191155 doi: 10.1242/dev.191155 Published 9 October 2020

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