Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Development
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Development

  • Log in
Advanced search

RSS  Twitter  Facebook  YouTube 

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Sign up for alerts
  • About us
    • About Development
    • About the Node
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contacts
    • Subscriptions
    • Feedback
RESEARCH ARTICLE
The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in rice
Christian A. Burr, Jinjing Sun, Maria V. Yamburenko, Andrew Willoughby, Charles Hodgens, Samantha Louise Boeshore, Agustus Elmore, Jonathan Atkinson, Zachary L. Nimchuk, Anthony Bishopp, G. Eric Schaller, Joseph J. Kieber
Development 2020 147: dev191734 doi: 10.1242/dev.191734 Published 27 October 2020
Christian A. Burr
1Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jinjing Sun
1Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jinjing Sun
Maria V. Yamburenko
2Department of Biological Sciences, Dartmouth College, Hanover, NH 03755
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew Willoughby
1Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Charles Hodgens
1Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
3Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Samantha Louise Boeshore
1Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Agustus Elmore
1Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan Atkinson
4School of Bioscience, University of Nottingham, Nottingham LE12 5RD, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zachary L. Nimchuk
1Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anthony Bishopp
4School of Bioscience, University of Nottingham, Nottingham LE12 5RD, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G. Eric Schaller
2Department of Biological Sciences, Dartmouth College, Hanover, NH 03755
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joseph J. Kieber
1Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
3Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Joseph J. Kieber
  • For correspondence: jkieber@bio.unc.edu

Handling Editor: Ykä Helariutta

  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF
Loading

ABSTRACT

The phytohormone cytokinin regulates diverse aspects of plant growth and development. Our understanding of the metabolism and perception of cytokinin has made great strides in recent years, mostly from studies of the model dicot Arabidopsis. Here, we employed a CRISPR/Cas9-based approach to disrupt a subset of cytokinin histidine kinase (HK) receptors in rice (Oryza sativa) in order to explore the role of cytokinin in a monocot species. In hk5 and hk6 single mutants, the root growth, leaf width, inflorescence architecture and/or floral development were affected. The double hk5 hk6 mutant showed more substantial defects, including severely reduced root and shoot growth, a smaller shoot apical meristem, and an enlarged root cap. Flowering was delayed in the hk5 hk6 mutant and the panicle was significantly reduced in size and infertile due to multiple defects in floral development. The hk5 hk6 mutant also exhibited a severely reduced cytokinin response, consistent with the developmental phenotypes arising from a defect in cytokinin signaling. These results indicate that HK5 and HK6 act as cytokinin receptors, with overlapping functions to regulate diverse aspects of rice growth and development.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: C.A.B., G.E.S., J.J.K.; Methodology: C.A.B., M.V.Y., J.J.K.; Formal analysis: C.A.B., J.S., M.V.Y., J.J.K.; Investigation: C.A.B., J.S., M.V.Y., A.W., C.H., S.L.B., A.E., J.A., A.B., J.J.K.; Resources: C.H., Z.L.N., J.J.K.; Writing - original draft: J.J.K.; Writing - review & editing: C.A.B., J.S., M.V.Y., A.W., Z.L.N., A.B., G.E.S., J.J.K.; Supervision: Z.L.N., A.B., G.E.S., J.J.K.; Project administration: G.E.S., J.J.K.; Funding acquisition: Z.L.N., G.E.S., J.J.K.

  • Funding

    This work was supported by the National Science Foundation (IOS-1238051 to G.E.S. and J.J.K.), the United States Department of Agriculture, Agriculture and Food Research Initiative, National Institute of Food and Agriculture program (2018-67013-27423 to J.J.K. and 2019-67013-29191 to G.E.S.), and the National Institutes of Health (R35GM119614) to Z.L.N. Deposited in PMC for release after 12 months.

  • Supplementary information

    Supplementary information available online at https://dev.biologists.org/lookup/doi/10.1242/dev.191734.supplemental

  • Received April 17, 2020.
  • Accepted September 23, 2020.
http://www.biologists.com/user-licence-1-1/
View Full Text

Log in using your username and password

Forgot your user name or password?

Log in through your institution

You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.
If your organization uses OpenAthens, you can log in using your OpenAthens username and password. To check if your institution is supported, please see this list. Contact your library for more details.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$30.00 .

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

Keywords

  • Cell signaling
  • Cytokinin
  • Plant development
  • Plant hormones

 Download PDF

Email

Thank you for your interest in spreading the word on Development.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in rice
(Your Name) has sent you a message from Development
(Your Name) thought you would like to see the Development web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
RESEARCH ARTICLE
The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in rice
Christian A. Burr, Jinjing Sun, Maria V. Yamburenko, Andrew Willoughby, Charles Hodgens, Samantha Louise Boeshore, Agustus Elmore, Jonathan Atkinson, Zachary L. Nimchuk, Anthony Bishopp, G. Eric Schaller, Joseph J. Kieber
Development 2020 147: dev191734 doi: 10.1242/dev.191734 Published 27 October 2020
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
RESEARCH ARTICLE
The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in rice
Christian A. Burr, Jinjing Sun, Maria V. Yamburenko, Andrew Willoughby, Charles Hodgens, Samantha Louise Boeshore, Agustus Elmore, Jonathan Atkinson, Zachary L. Nimchuk, Anthony Bishopp, G. Eric Schaller, Joseph J. Kieber
Development 2020 147: dev191734 doi: 10.1242/dev.191734 Published 27 October 2020

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • Acknowledgements
    • Footnotes
    • References
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF

Related articles

Cited by...

More in this TOC section

  • Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root development
  • Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish
  • Deciphering and modelling the TGF-β signalling interplays specifying the dorsal-ventral axis of the sea urchin embryo
Show more RESEARCH ARTICLE

Similar articles

Subject collections

  • Plant development

Other journals from The Company of Biologists

Journal of Cell Science

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Kathryn Virginia Anderson (1952-2020)

Developmental geneticist Kathryn Anderson passed away at home on 30 November 2020. Tamara Caspary, a former postdoc and friend, remembers Kathryn and her remarkable contribution to developmental biology.


Zooming into 2021

In a new Editorial, Editor-in-Chief James Briscoe and Executive Editor Katherine Brown reflect on the triumphs and tribulations of the last 12 months, and look towards a hopefully calmer and more predictable year.


Read & Publish participation extends worldwide

Over 60 institutions in 12 countries are now participating in our Read & Publish initiative. Here, James Briscoe explains what this means for his institution, The Francis Crick Institute. Find out more and view our full list of participating institutions.


Upcoming special issues

Imaging Development, Stem Cells and Regeneration
Submission deadline: 30 March 2021
Publication: mid-2021

The Immune System in Development and Regeneration
Guest editors: Florent Ginhoux and Paul Martin
Submission deadline: 1 September 2021
Publication: Spring 2022

Both special issues welcome Review articles as well as Research articles, and will be widely promoted online and at key global conferences.


Development presents...

Our successful webinar series continues into 2021, with early-career researchers presenting their papers and a chance to virtually network with the developmental biology community afterwards. Sign up to join our next session:

10 February
Time: 13:00 (GMT)
Chaired by: preLights

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Sign up for alerts

About us

  • About Development
  • About the Node
  • Editors and board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • Biology Open transfer

Journal info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact Development
  • Subscriptions
  • Advertising
  • Feedback

 Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992