PT  - JOURNAL ARTICLE
AU  - Friesel, R.
AU  - Brown, S.A.
TI  - Spatially restricted expression of fibroblast growth factor receptor-2 during Xenopus development
DP  - 1992 Dec 01
TA  - Development
PG  - 1051--1058
VI  - 116
IP  - 4
4099  - http://dev.biologists.org/content/116/4/1051.short
4100  - http://dev.biologists.org/content/116/4/1051.full
SO  - Development1992 Dec 01; 116
AB  - The fibroblast growth factors (FGFs) play a role in Xenopus laevis embryonic development, particularly in the induction of ventral-type mesoderm. We have isolated a full-length cDNA from Xenopus that we have designated Xenopus fibroblast growth factor receptor-2 (XFGFR-2), with significant amino acid sequence similarity to the previously described bek gene (FGFR-2). We expressed the XFGFR-2 cDNA in COS1 cells and showed that it functions as an FGF receptor by binding radiolabeled FGF-2. RNA gel blot analysis demonstrates that unlike Xenopus fibroblast growth factor receptor-1 (XFGFR-1), XFGFR-2 mRNA expression begins during gastrulation and continues through early tadpole stages. Whole-mount in situ hybridization demonstrates that XFGFR-2 mRNA is localized to the anterior neural plate in early neurula stage embryos. Later in development, XFGFR-2 expression is found in the eye anlagen, midbrain-hindbrain boundary and the otic vesicle. In addition, XFGFR-2 transcripts are expressed in animal caps in a manner that is independent of mesoderm-inducing factors. These results indicate that XFGFR-2 may have a role in development that is distinct from that of XFGFR-1.