PT  - JOURNAL ARTICLE
AU  - Xu, Q.
AU  - Alldus, G.
AU  - Holder, N.
AU  - Wilkinson, D.G.
TI  - Expression of truncated Sek-1 receptor tyrosine kinase disrupts the segmental restriction of gene expression in the Xenopus and zebrafish hindbrain
DP  - 1995 Dec 01
TA  - Development
PG  - 4005--4016
VI  - 121
IP  - 12
4099  - http://dev.biologists.org/content/121/12/4005.short
4100  - http://dev.biologists.org/content/121/12/4005.full
SO  - Development1995 Dec 01; 121
AB  - During development of the vertebrate hindbrain regulatory gene expression is confined to precise segmental domains. Studies of cell lineage and gene expression suggest that establishment of these domains may involve a dynamic regulation of cell identity and restriction of cell movement between segments. We have taken a dominant negative approach to interfere with the function of Sek-1, a member of the Eph-related receptor tyrosine kinase family expressed in rhombomeres r3 and r5. In Xenopus and zebrafish embryos expressing truncated Sek-1, lacking kinase sequences, expression of r3/r5 markers occurs in adjacent even-numbered rhombomeres, in domains contiguous with r3 or r5. This disruption is rescued by full-length Sek-1, indicating a requirement for the kinase domain in the segmental restriction of gene expression. These data suggest that Sek-1, perhaps with other Eph-related receptors, is required for interactions that regulate the segmental identity or movement of cells.