PT  - JOURNAL ARTICLE
AU  - Alexandre, Paula
AU  - Wassef, Marion
TI  - The isthmic organizer links anteroposterior and dorsoventral patterning in the mid/hindbrain by generating roof plate structures
AID  - 10.1242/dev.00756
DP  - 2003 Nov 15
TA  - Development
PG  - 5331--5338
VI  - 130
IP  - 22
4099  - http://dev.biologists.org/content/130/22/5331.short
4100  - http://dev.biologists.org/content/130/22/5331.full
SO  - Development2003 Nov 15; 130
AB  - During vertebrate development, an organizing signaling center, the isthmic organizer, forms at the boundary between the midbrain and hindbrain. This organizer locally controls growth and patterning along the anteroposterior axis of the neural tube. On the basis of transplantation and ablation experiments in avian embryos, we show here that, in the caudal midbrain, a restricted dorsal domain of the isthmic organizer, that we call the isthmic node, is both necessary and sufficient for the formation and positioning of the roof plate, a signaling structure that marks the dorsal midline of the neural tube and that is involved in its dorsoventral patterning. This is unexpected because in other regions of the neural tube, the roof plate has been shown to form at the site of neural fold fusion, which is under the influence of epidermal ectoderm derived signals. In addition, the isthmic node contributes cells to both the midbrain and hindbrain roof plates, which are separated by a boundary that limits cell movements. We also provide evidence that mid/hindbrain roof plate formation involves homeogenetic mechanisms. Our observations indicate that the isthmic organizer orchestrates patterning along the anteroposterior and the dorsoventral axis.