PT  - JOURNAL ARTICLE
AU  - Kuwajima, Takaaki
AU  - Sitko, Austen A.
AU  - Bhansali, Punita
AU  - Jurgens, Chris
AU  - Guido, William
AU  - Mason, Carol
TI  - <em>Clear<sup>T</sup></em>: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue
AID  - 10.1242/dev.091844
DP  - 2013 Mar 15
TA  - Development
PG  - 1364--1368
VI  - 140
IP  - 6
4099  - http://dev.biologists.org/content/140/6/1364.short
4100  - http://dev.biologists.org/content/140/6/1364.full
SO  - Development2013 Mar 15; 140
AB  - We describe a clearing method for enhanced visualization of cell morphology and connections in neuronal and non-neuronal tissue. Using ClearT or ClearT2, which are composed of formamide or formamide/polyethylene glycol, respectively, embryos, whole mounts and thick brain sections can be rapidly cleared with minimal volume changes. Unlike other available clearing techniques, these methods do not use detergents or solvents, and thus preserve lipophilic dyes, fluorescent tracers and immunohistochemical labeling, as well as fluorescent-protein labeling.