RT Journal Article
SR Electronic
T1 A targeted gene expression system using the tryptophan repressor in zebrafish shows no silencing in subsequent generations
JF Development
JO Development
FD The Company of Biologists Limited
SP 1167
OP 1174
DO 10.1242/dev.100057
VO 141
IS 5
A1 Suli, Arminda
A1 Guler, Ali D.
A1 Raible, David W.
A1 Kimelman, David
YR 2014
UL http://dev.biologists.org/content/141/5/1167.abstract
AB The ability to visualize and manipulate cell fate and gene expression in specific cell populations has made gene expression systems valuable tools in developmental biology studies. Here, we describe a new system that uses the E. coli tryptophan repressor and its upstream activation sequence (TrpR/tUAS) to drive gene expression in stable zebrafish transgenic lines and in mammalian cells. We show that TrpR/tUAS transgenes are not silenced in subsequent generations of zebrafish, which is a major improvement over some of the existing systems, such as Gal4/gUAS and the Q-system. TrpR transcriptional activity can be tuned by mutations in its DNA-binding domain, or silenced by Gal80 when fused to the Gal4 activation domain. In cases in which more than one cell population needs to be manipulated, TrpR/tUAS can be used in combination with other, existing systems.