PT  - JOURNAL ARTICLE
AU  - Rui, Menglong
AU  - Bu, Shufeng
AU  - Chew, Liang Yuh
AU  - Wang, Qiwei
AU  - Yu, Fengwei
TI  - The membrane protein Raw regulates dendrite pruning via the secretory pathway
AID  - 10.1242/dev.191155
DP  - 2020 Oct 01
TA  - Development
PG  - dev191155
VI  - 147
IP  - 19
4099  - http://dev.biologists.org/content/147/19/dev191155.short
4100  - http://dev.biologists.org/content/147/19/dev191155.full
SO  - Development2020 Oct 01; 147
AB  - Neuronal pruning is essential for proper wiring of the nervous systems in invertebrates and vertebrates. Drosophila ddaC sensory neurons selectively prune their larval dendrites to sculpt the nervous system during early metamorphosis. However, the molecular mechanisms underlying ddaC dendrite pruning remain elusive. Here, we identify an important and cell-autonomous role of the membrane protein Raw in dendrite pruning of ddaC neurons. Raw appears to regulate dendrite pruning via a novel mechanism, which is independent of JNK signaling. Importantly, we show that Raw promotes endocytosis and downregulation of the conserved L1-type cell-adhesion molecule Neuroglian (Nrg) prior to dendrite pruning. Moreover, Raw is required to modulate the secretory pathway by regulating the integrity of secretory organelles and efficient protein secretion. Mechanistically, Raw facilitates Nrg downregulation and dendrite pruning in part through regulation of the secretory pathway. Thus, this study reveals a JNK-independent role of Raw in regulating the secretory pathway and thereby promoting dendrite pruning.