RT Journal Article
SR Electronic
T1 The membrane protein Raw regulates dendrite pruning via the secretory pathway
JF Development
JO Development
FD The Company of Biologists Limited
SP dev191155
DO 10.1242/dev.191155
VO 147
IS 19
A1 Rui, Menglong
A1 Bu, Shufeng
A1 Chew, Liang Yuh
A1 Wang, Qiwei
A1 Yu, Fengwei
YR 2020
UL http://dev.biologists.org/content/147/19/dev191155.abstract
AB Neuronal pruning is essential for proper wiring of the nervous systems in invertebrates and vertebrates. Drosophila ddaC sensory neurons selectively prune their larval dendrites to sculpt the nervous system during early metamorphosis. However, the molecular mechanisms underlying ddaC dendrite pruning remain elusive. Here, we identify an important and cell-autonomous role of the membrane protein Raw in dendrite pruning of ddaC neurons. Raw appears to regulate dendrite pruning via a novel mechanism, which is independent of JNK signaling. Importantly, we show that Raw promotes endocytosis and downregulation of the conserved L1-type cell-adhesion molecule Neuroglian (Nrg) prior to dendrite pruning. Moreover, Raw is required to modulate the secretory pathway by regulating the integrity of secretory organelles and efficient protein secretion. Mechanistically, Raw facilitates Nrg downregulation and dendrite pruning in part through regulation of the secretory pathway. Thus, this study reveals a JNK-independent role of Raw in regulating the secretory pathway and thereby promoting dendrite pruning.