Table 1.

Effect of morpholino antisense oligonucleotides

Amount of morpholino antisense oligonucleotides injected per embryo
hrTMO(1) Control-MO hrTMO(2)
Observed phenotypes1.5 ng1.5 ng6.0 ng8.0 ng10.0 ng12.5 ng
Dysmorphic heart78%2%30%44%69%93%
No circulating blood65%4%15%38%47%79%
Normal cardiovascular development12%94%70%56%31%7%
Total embryo number10098112102179109
  • These phenotype frequencies (%) are a representative of two or three experiments. Analysis was at 38-42 hpf.