Table 2.

Effect of spaw knockdown on organ laterality

Heart
A Cardiac joggingGroupn% L Jog% R Jog% No Jog
NC-MO2810000
spaw-MO127521930
NC-MO3278220
spaw-MO22836757
Uninjected509820
Cyc-MO239190
B Jogging versus looping Group n Jog% Jog% D-loop% L-loop% No loop
Uninjected52Left jog10010000
Right jog0000
No jog0000
NC-MO57Left jog949801
Right jog501000
No jog0000
spaw-MO155Left jog5156538
Right jog20502030
No jog28392040
Pancreas
C Position of pancreas Group n %R%L%M
NC-MO4372721
spaw-MO143302149
  • n, number of embryos; % jog, % of the number of embryos with a left, right or no jog phenotype; % D-loop, the fraction of embryos of a given jogging phenotype that subsequently developed a D-loop. Likewise for % L-loop and % No loop; %R, %L or %M, % of injected embryos with the pancreas positioned to the right or left of the midline or on the midline, respectively; Cyc-MO, cyclops morpholino; NC-MO, Gene Tools negative control morpholino; spaw-MO1, southpaw morpholino complementary to translation start site; spaw-MO2, southpaw morpholino complementary to a splice acceptor site.

    The dose of Cyc-MO was 8 ng per embryo. For all other morpholinos, the dose was 10 ng per embryo.

    Results shown in B are pooled from two morpholino experiments; results in C are pooled from three morpholino experiments.