Table 3.

Effect of spaw knockdown on asymmetric gene expression

Lateral plate expression (%) Dorsal diencephalon (%)
GeneStage for in situMorpholinonLeftRightBilAbsentLeftAbsent
19-22 sNone448400166434
lefty1 19-22 sNC-MO29930076634
19-22 sspaw-MO129000100394
21-23 sNone306000408020
lefty1 21-23 sNC-MO264240568119
21-23 sspaw-MO227700931189
cyclops 18-20 sNC-MO21865096729
18-20 sspaw-MO120500950100
None361400861000
cyclops 18-22 sNC-MO40200080955
18-22 sspaw-MO1401700831585
22-24 sNone4295005982
pitx2 22-24 sNC-MO321000001000
22-24 sspaw-MO13630097694
16-18 sNone3692080
southpaw 16-18 sNC-MO47832114
16-18 sspaw-MO146220078
18-20 sNone34100000
southpaw 18-20 sNC-MO2195005
18-20 sspaw-MO21950095
19-23 sNone42810019
lefty2 19-23 sNC-MO38790021
19-23 sspaw-MO141000100
  • s, somites; n, number of embryos injected; %, % of injected embryos with expression of a given marker gene in a given territory; Bil, bilateral.

    MO dose was 10 ng per embryo.

    Lateral plate expression of pitx2 was evaluated by monitoring changes in left-side pitx2c expression near the posterior gut.