Table 1.

PCR primer pairs and PCR cycling conditions used with the LightCycler™

ααPCR primer pairOriginSequenceDenat. temp.Annealing temp (°C)/ time (sec)Extension temp (°C)/ time (sec)Acquisition temp (°C)/ time (sec)
Xbra Sun et al., 1999 U: 5-TTC TGA AGG TGA GCA TGT CG-39555/572/875/3
D: 5-GTT TGA CTT TGC TAA AAG AGA CAG G-3
cerberus Heasman et al., 2000 U: 5-GCT TGC AAA ACC TTG CCC TT-39560/572/2081/3
D: 5-CTG ATG GAA CAG AGA TCT TG-3
chordin XMMRU: 5-AAC TGC CAG GAC TGG ATG GT-39555/572/1281/3
D: 5-GGC AGG ATT TAG AGT TGC TTC-3
eFGF Casey et al., 1998 U: 5-CTT TCT TTC CAG AGA AAC GAC ACC G-39560/572/1283/3
D: 5-AAC TCA CGA CTC CAA CTT CCA CTG-3
en2 NewU: 5-AAG CAA ACC AAA CAT TTG CC-39560/572/580/3
D: 5-ATC CAT AAA CAG GGA AGG GG-3
NCAMNewU: 5-CAC AGT TCC ACC AAA TGC-39560/572/1384/3
D: 5-GGA ATC AAG CGG TAC AGA-3
Xnrp1 Lamb et al., 1995U: 5-GGG TTT CTT GGA ACA AGC-39555/572/1484/3
D: 5-ACT GTG CAG GAA CAC AAG-3
ODC Heasman et al., 2000 U: 5-GCC ATT GTG AAG ACT CTC TCC ATT C-39555/572/1283/3
D: 5-TTC GGG TGA TTC CTT GCC AC-3
Pax6 NewU: 5-CGA TGG GCA ACA ATC TAC-39555/572/1084/3
D: 5-GAC TGA CAC TCC AGG GGA-3
XlHbox6NewU: 5-TAC TTA CGG GCT TGG CTG GA-39558/572/887/3
D: 5-AGC GTG TAA CCA GTT GGC TG-3
MyoD Rupp et al. 1991U: 5-AGC TCC AAC TGC TCC GAC GGC ATG AA-39555/572/1886/3
D: 5-AGG AGA GAA TCC AGT TGA TGG AAA CA-3
eomesoderminNewU: 5-TGG TCC TCA AGG TCA AGT CC-39556/672/1083/3
D: 5-GGG GAG TTT TCA TTG CTT GA-3