Table 1.

Timing of developmental and behavioral events at ecdysis from 2nd to 3rd instar larva in KO animals

EventKO Control
DMP - cuticle shed36.29±0.9131.89±1.01 (*)
DMP - tracheal air entry16.71±0.9716.41±0.81 (ns)§
Tracheal air entry - pre-ecdysis start2.09±0.613.33±0.77 (ns)
Pre-ecdysis start - end14.25±1.0111.00±0.86 (*)
No. of pre-ecdysis contractions99.1±5.780.6±7.1 (**)
Frequency of pre-ecdysis contractions7.11±0.497.40±0.43 (ns)
Ecdysis `bites' - cuticle shed3.24±0.531.15±0.07 (**)
Ecdysis `bites' - P/A peristalses 1.78±0.390.64±0.08 (**)
P/A peristalses - cuticle shed1.46±0.420.51±0.06 (*)
No. of ecdysis `bites'64.9±12.042.2±4.1 (*)
No. of A/P peristalses ††4.4±1.60.0±0.0 (**)
No. of P/A peristalses8.7±0.93.7±0.2 (**)
  • Values are averages±s.e.m.; n=8 for each group. Units are minutes or number, depending on the event.

  • Double Mouth Plate stage.

  • § (ns) P>0.05;

  • (*) 0.01<P<0.05;

  • (**) P<0.01. Student's t-test.

  • P/A peristalses: Anteriorly directed peristaltic waves.

  • †† A/P peristalses: Posteriorly directed peristaltic waves.