Table 1.

Potential Sox binding sites in the nac/mitfa promoter and mutated versions

Position in promoterOligonucleotides used to test Sox 10 binding
-157 b.p.S15′ccatcgtCTCAAAGaggctt3′
3′gtagcaGAGTTTCtccgaaa5′
M1 (mutated S1)5′ccatcgtCGGCGGTaggctt3′
3′gtagcaGCCGCCAtccgaaa5′
-247 b.p.S25′gagaacaAACAATGttttat3′
3′tcttgtTTGTTACaaaatac5′
M2 (mutated S2)5′gagaacaACCGCGGttttat3′
3′tcttgtTGGCGCCaaaatac5′
-262 b.p.S35′tgctagtGATTGTAtgccgg3′
3′cgatcaCTAACATacggcct5′
M3 (mutated S3)5′tgctagtGGTACCAtgccgg3′
3′cgatcaCCATGGTacggcct5′
-284 b.p.S45′ttagaccAACAGTGctagtg3′
3′atctggTTGTCACgatcact5′
M4 (mutated S4)5′ttagaccACCCGGGctagtg3′
3′atctggTGGGCCCgatcact5′