Table 3.

GFP expression in wild type embryos from a nac/mitfa promoter construct is reduced by mutating a Cls/Sox 10-binding site in the promoter of the plasmid

Injected plasmid *Number of embryos injectedNumber of embryos with GFP fluorescent crest cells at 24 hpf (%)
nac>nac13287 (66)
M1nac>nac17929 (16)
M3nac>nac13955 (40)
M1M3nac>nac15310 (6.5)
  • * 25 pg plasmid injected per embryo.