Table 2.

UAS-skd and UAS-kto transgenes can rescue the lethality of the corresponding mutations

% rescue to pupal stage Number
skd rescue
skdT13, da-GAL4/TM6B×skdT606/TM6B0262
skdT13/TM6B×skdT606, UASskd1/TM6B11203
skdT13; tub-GAL4/SM6. TM6B×skdT606, UASskd1/TM6B63246
skdT13, da-GAL4/TM6B×skdT606, UASskd1/TM6B58291
skdT13/TM6B×skdT413; UASskd3/SM6. TM6B0156
skdT13; tub-GAL4/SM6. TM6B×skdT413; UASskd3/SM6. TM6B54128
skdT13, da-GAL4/TM6B×skdT413; UASskd3/SM6. TM6B109252
kto rescue
ktoT555, da-GAL4/TM6B×ktoT241/TM6B0535
ktoT555/TM6B×ktoT241; UASkto1/SM6. TM6B0413
ktoT555, da-GAL4/TM6B×ktoT241; UASkto1/SM6. TM6B80381
  • For each cross, the percentage rescue to the pupal stage is given, based on comparing the number of non-balancer pupae with the number of balancer adults that eclosed. The TM6B and SM6-TM6B balancers carried the Humeral and Tubby markers. UAS-skd1 showed some leaky activity in the absence of a GAL4 driver, and all UAS lines caused some early lethality when expressed with the strong driver da-GAL4, preventing complete rescue.