Table 1.

Primitive and definitive progenitors derived from CD41bright, CD41dimand CD41lo/-cells from murine embryos

DayEry-PCFU-MacCFU-MegBFU-ECFU-mixCFU-GM
E7.0
CD41brightNDNPNPNPNPNP
CD41dim6.2±4.1NPNPNPNPNP
CD41lo/-0NPNPNPNPNP
E7.5
CD41brightNPNPNDNPNPNP
CD41dimNPNP4.1±2.7NPNPNP
CD41lo/-NPNP0NPNPNP
E8.0
CD41brightNPNDNPNPNPNP
CD41dimNP8.6±2.3NPNPNPNP
CD41lo/-NP0NPNPNPNP
E8.25
CD41bright0.5±0.70.1±0.23.3±0.96.0±1.414.0±2.89.0±2.0
CD41dim14.8±4.812.3±3.40.2±0.30.3±0.51.7±1.60.8±1.0
CD41lo/-0.8±1.200000
  • Primitive erythroid (Ery-P), early appearing macrophage (CFU-Mac) and megakaryocyte (CFU-Meg) and definitive progenitor cells (BFU-E, CFU-Mix, CFU-GM) were enumerated from cultures initiated with 1000 sorted cells.

    Data presented as mean±s.d. in 2-4 experiments.

    NP, not performed and ND, not detectable by flow cytometry.