Day | Ery-P | CFU-Mac | CFU-Meg | BFU-E | CFU-mix | CFU-GM |
---|---|---|---|---|---|---|
E7.0 | ||||||
CD41^{bright} | ND | NP | NP | NP | NP | NP |
CD41^{dim} | 6.2±4.1 | NP | NP | NP | NP | NP |
CD41^{lo/-} | 0 | NP | NP | NP | NP | NP |
E7.5 | ||||||
CD41^{bright} | NP | NP | ND | NP | NP | NP |
CD41^{dim} | NP | NP | 4.1±2.7 | NP | NP | NP |
CD41^{lo/-} | NP | NP | 0 | NP | NP | NP |
E8.0 | ||||||
CD41^{bright} | NP | ND | NP | NP | NP | NP |
CD41^{dim} | NP | 8.6±2.3 | NP | NP | NP | NP |
CD41^{lo/-} | NP | 0 | NP | NP | NP | NP |
E8.25 | ||||||
CD41^{bright} | 0.5±0.7 | 0.1±0.2 | 3.3±0.9 | 6.0±1.4 | 14.0±2.8 | 9.0±2.0 |
CD41^{dim} | 14.8±4.8 | 12.3±3.4 | 0.2±0.3 | 0.3±0.5 | 1.7±1.6 | 0.8±1.0 |
CD41^{lo/-} | 0.8±1.2 | 0 | 0 | 0 | 0 | 0 |
Primitive erythroid (Ery-P), early appearing macrophage (CFU-Mac) and megakaryocyte (CFU-Meg) and definitive progenitor cells (BFU-E, CFU-Mix, CFU-GM) were enumerated from cultures initiated with 1000 sorted cells.
Data presented as mean±s.d. in 2-4 experiments.
NP, not performed and ND, not detectable by flow cytometry.