Table 1.

Primer sets used for PCR analysis

Target geneUpstream primer (5′ to 3′)Downstream primer (5′ to 3′)Cycle numberNumber of bpAccession number/reference
ET CCT ATC CTT GAC TTG CTA CAGTT TTG GGG AAG GAG GGT AT24255AF173940
Pax6 GCA ACC TGG CGA GCG ATA AGCCCT GCC GTC TCT GGT TCC GTA GTT28450U76386
Six3 TTG TCT GTC TGT CTC TTG TTTTC TGT GTT TGG TTT ATC TC30369AF183571
Rx1 CCC CAA CAG GAG CAT TTA GAA GACAGG GCA CTC ATG GCA GAA GGT T28416AF017273
tll ACT TGC CTC TCG TGC TGC TCT ACT GATC CGG TCG GGT TGC TCA TCT T30351U67886
Lhx2 ACC CTC CTC CCC CAT TAC TCA CAGG GCA TAT CTG GGC ATC TTC A30461AY141037
Optx2 ACA GAG CAG CGG CGG CAA AGAGAG CGC TCC CTG GTA CTG TGA CTG A30296AF081352
NCAM CAC AGT TCC ACC AAA TGCGGA ATC AAG CGG TAC AGA30343Xenbase
Otx2 GGA TGG ATT TGT TAC ATC CGT CCAC TCT CCG AGC TCA CTT CCC25315U19813
XAG GAC TGG TGC TGT TCA ACC TTGCAT TGG GAA ATA ACT GGG ACC25349U76752
H4 CGG GAT AAC ATT CAG GGT ATC ACTATC CAT GGC GGT AAC TGT CTT CCT24189Hollemann et al., 1998
  • Primer sets were designed from the indicated GenBank sequence or from the indicated source (see Materials and methods for the details of the PCR reaction conditions). Xenbase primer sequences can be found at http://www.xenbase.org/