Table 1.

Colony formation from early tissues

Cell sourceCFU-GEMMBFU-ECFU-GM
E8
    5 spP000
YS000
CH000
    9 spP000
YS000
CH000
E8.5
    15 spP000
YS2.5±0.61.75±0.50
CH000
    18 spP0.5±0.500.5±0.5
YS3.0±1.52.5±0.50
CH3.5±1.50.5±1.02.5±2.0
E9
    20 spP4.5±1.01.0±0.52.5±1.0
YS5.0±1.04.0±1.02.0±0.5
CH6.0±1.51.0±1.55.0±1.0
  • Table shows number of GFP+ colonies/104 cells.

    104 cells were cultured in the presence of growth factors (SCF, IL3, IL6, EPO). Colonies were scored at 14 days of culture.

    Each value is the means±s.d. of four assays (four wells per assay).

    P, placenta; YS, yolk sac; CH, caudal halves of embryos; FL, fetal liver; sp, somite pairs.