Table 1.

Distribution of the segmentation defects in embryos injected with dally or dally-like dsRNA

TreatmentnSegmentation defects (total)Weak segmentation defectsStrong segmentation defectsLawn of denticles
Buffer9914 (14%)14 (14%)00
dally RNAi22635 (15%)35 (15%)00
dally-like RNAi211193 (91%)60 (28%)46 (22%)87 (41%)
  • Embryos were injected with either buffer, dally or dally-like dsRNA, and the cuticle of the ventral abdomen scored for segmentation defects at the end of embryogenesis. Only embryos with an intact cuticle in the trunk were analysed (n=total number of analysed embryos). Segmentation defects were classified into three categories. In `lawn of denticles' and `strong segmentation defects', every segment had transformations of naked cuticle into denticles, with some naked cuticle remaining in embryos of the `strong segmentation defects' category. The other embryos with segmentation defects were categorised as weak. Only weak segmentation defects were found after injection of dally dsRNA or buffer. dally RNAi was performed using the 5′, middle or 3′ dsRNA sequences. The same proportion of weak segmentation defects were observed in each case and the results have been added together for this table. For dally-like, injections were performed with a 5′ dsRNA or a 3′dsRNA. The same distribution of weak, strong and lawn phenotypes were observed (not shown). The table shows the result of a typical experiment using the 3′ dsRNA sequence, which is the dsRNA used for the RNAi experiments presented in Figs 2, 3, 4, 5, 6.