Table 1.

Oligos used in this study

Oligo sequence: restriction sites are underlinedComments
Sense primer
5′-gcgGGTACCagatctaaccattgattaac, KpnIBam promoter -898
5′-gcgGGTACCttcaagaattttgtaaccga, KpnIBam promoter -799
5′-gcgACGCGTttcaagaattttgtaaccga, MluIBam promoter -799
5′-gcgGGTACCaaaattttgctgcgtcagtc, KpnIBam promoter -698
5′-gcgGGTACCacggagctgccagatgttag, KpnIBam promoter -498
5′-gcgGGTACCccaaatcagtgtgtataatt, KpnIBam promoter -198
5′-gcgGGTACCtgtaaagttcgtaatggatt, KpnIBam promoter -168
5′-gcgGGTACCaggtgaccataaattgaaac, KpnIBam promoter -96
5′-gcgACGCGTaggtgaccataaattgaaac, MluIBam promoter -96
5′-gcgCTCGAGtgaaacttatttgtgcgacg XhoIBam promoter -82
5′-gcgGGTACCtactcgacatgatatcgata, KpnIBam promoter -47
5′-gcgAAGCTTaaccctgttctgggtactcga, HindIIIBam promoter -60
5′-gcgGGTACCaaagagtctggacgccatca, KpnIBam promoter -16
5′-gcgACGCGTttcttcctctttctcctgaa, MluIBam promoter +5
5′-gcgACGCGTagacagcgtggcgtcagcga, MluIBam promoter +30
5′-gcgGGTACCcgaccgttttaacctcgaaa, KpnINanos promoter -708
5′-gcgACGCGTactacgcgtgtcgatagccg, MluIP-element basal promoter
Antisense primer
5′-gcgGGATCCtaagttaaatcacacaaatc, BamHIBam promoter +133
5′-gcgACCGGTcgatttttgccttttaaatc, AgeIBam promoter +104
5′-gcgACCGGTttatcgccaggttcttttta, AgeIBam promoter +79
5′-gcgACCGGTtgaaatcgctgacgccacgc, AgeIBam promoter +55
5′-gcgACCGGTgacgccacgctgtctgcgaa, AgeIBam promoter +45
5′-gcgACCGGTtgtctgcgaattcaggagaa, AgeIBam promoter +34
5′-gcgACCGGTgcgaattcaggagaaagagg, AgeIBam promoter +30
5′-gcgACCGGTtgatggcgtccagactattt, AgeIBam promoter +4
5′-gcgACCGGTtaacgtatcgatatcatgtc, AgeIBam promoter -20
5′-gcgACGCGTtaacgtatcgatatcatgtc, MluIBam promoter -20
5′-gcgACCGGTcccagaacaggttgccgtc, AgeIBam promoter -47
5′-gcgAAGCTTcaatttatgctcacctcaaaa, HindIIIBam promoter -81
5′-gcgAAGCTTacaaataagtttcaatttatg, HindIIIBam promoter -69
5′-gcgACCGGTtgaaatcgctgacgcgctgtctgc, AgeIBam promoter +55 with deletion of +37 to +39
5′-gcgACCGGTtgtctgattcaggagaaagag, AgeIBam promoter +34 with deletion of +20∼22
5′-cgccacgctgtctgcgaataacgtatcgatatcatBam promoter -38 to -22/+24 to +42
5′-gcgACCGGTcgctgtctgcgaataacgtatcgatatcat, AgeIBam promoter -38 to -22/+24 to +37
5′-ctgacgccaaattcaggagaaagaggaagBam promoter +7 to +26/+38 to +46
5′-gcgACCGGTttgaaatcgctgacgccaaattcaggagaa, AgeIBam promoter +55 with deletion +27 to +37
5′-gcgCTCGAGcaattccaggaattttgtgg, XhoINanos promoter -1
5′-gcgACCGGTgtaactaaactcgcttttgg, AgeINanos promoter +94
5′-cgcACCGGTattccacgtaagggttaatg, AgeIP-element basal promoter