Table 1.

Strains used in this study

Strain nameDescriptionGenotype
JH227PIE-1:GFP axEx73[pJH3.92 PIE-1:GFP; pRF4]
JH1447GFP:MEX-6 axEx1124[pKR2.07 GFP:MEX-6; pRF4]
JH1448GFP:MEX-5 axEx1125[pKR2.04 GFP:MEX-5; pRF4]
JH1512GFP:PAR-6 axIs1137[pJH7.04 GFP:PAR-6; pRF4]
JH1513PIE-1:GFP in par-3 mutant par-3(it71) unc-32(e189)/qC1; axEx73[pJH3.92 PIE-1:GFP; pRF4]
JH1528PIE-1:GFP in par-1 mutant par-1(ax53) dpy-11 (e224)/nT1 V; axEx73[pJH3.92 PIE-1:GFP; pRF4]
KK866GFP:PAR-2 itIs153[pMW1.03 GFP:PAR-2; pRF4]
JH1473GFP:PAR-2 and GFP:tubulinitIs153[pMW1.03 GFP:PAR-2; pRF4];ojIs1[pJH4.66 w/unc-119 inserted at NaeI]
JJ1244mex-5;mex-6 double mutant mex-6(pk440); unc-30(e191) mex-5(zu199) IV/nT1
KK747par-2 mutant par-2(lw32) unc-45(e286ts)/sC1 III