Table 1.

Quantification of cell proliferation in the hindbrain of hdac1hi1618 mutant and unaffected sibling embryos at 25, 33 and 38 hpf

Age
25 hpf33 hpf38 hpf
hdac1hi1618 siblings285±11.5306±2.9300±17.8
hdac1hi1618 mutants176±14.6320±14.3288±8.6
  • The total number of darkly stained phospho-H3-positive nuclei in the region of the hindbrain spanning rhombomeres 2-7 was counted under the compound microscope. Values given are mean cell counts (±s.e.m.) where n=4 individual embryos in each sample group (see Fig. 3 for examples).