Table 1.

Cyclopamine reduces muscle differentiation in tail somites

ExperimentCyclopamine treatmentAnalysisNumber of somites expressing 12/101*Number of somites expressing 12/101 across entire dorsoventral extent without a gap*
1Stage 12Stage 3630±1 (3)13±2 (3)
ControlStage 3632±2 (3)32±2 (3)
Stage 12Stage 48∼45 (3)∼35 (3)
ControlStage 48∼50 (3)∼50 (3)
2Stage 22-26Stage 4130.7±2.4 (10)30.0±2.1 (10)
ControlStage 4140.4±3.3 (18)40.4±3.3 (18)
Stage 22-26Stage 4848.6±3.2 (11)31.3±2.1 (11)
ControlStage 4853.3±2.4 (13)50.0±2.4 (13)
3Stage 21Stage 4741.7±0.7 (3)29.7±0.6 (3)
ControlStage 4746.3±2.1 (3)37.3±0.6 (3)
  • Whole-mount stained embryos were scored under a Zeiss Axiophot.

    Control, ethanol vehicle control slightly retards development but muscle appears normal.

  • * Mean±s.d. (number of embryos)

  • Somite counts inaccurate because muscle was hypercontracted