Table 3.

Proportion of Ngn2-positive cells out of the total surface mitoses or non-surface mitoses

Surface divisionNon-surface division
Ngn2-positive/p-vim-positive4%32%
Ngn2-positive/Ki67 (+++)5%44%
  • Sections collected from six embryos at E13 or E14 were examined using a confocal microscope (40× or 100× objective).

    Total number of p-vim-positive cells counted: 406 cells for S division, 234 cells for NS division.

    Total number of Ki67+++ cells counted: 205 cells for S division, 143 cells for NS division.

    Differences between the S and NS groups were significant (P<0.0001, chisquare test) in both detection methods.