Table 2.

DAF-16 regulates daf-15 expression in vivo

Excretory Cells
StrainWeak*MediumStrongMusclesIntestineNeuronsHypo§DTCn**
Wild-type GFP reporter
   Transgenic line 1
   daf-28502000050
   daf-2 (daf-16 RNAi)19613610050
   Transgenic line 2
   daf-28412200050
   daf-2 (daf-16 RNAi)171527842150
IRS mutant GFP reporter
   Transgenic line 3
   daf-22101000050
   daf-2 (daf-16 RNAi)6333433050
   Transgenic line 4
   daf-23011000050
   daf-2 (daf-16 RNAi)12611100050
  • * A weak signal in the excretory cell body

  • A weak signal in both excretory cell body and canals or a clear signal in the excretory cell body

  • A strong signal in both excretory cell body and canals

  • § Hypodermis cells

  • Distal tip cells

  • ** Total number of animals scored