Table 1.

PCR primer pairs and PCR cycling conditions used with the Lightcycler™

PCR primer pairOriginSequenceDenaturing temperature (°C)Annealing temperature (°C)/time (sec)Extension temperature (°C)/time (sec)Acquisition temperature (°C)/time (sec)
Chordin XMMR*U: 5′-AACTGCCAGGACTGGATGGT-3′9555/572/1281/3
D: 5′-GGCAGGATTTAGAGTTGCTTC-3′
Cerberus Heasman et al., 2000U: 5′-GCTTGCAAAACCTTGCCCTT-3′9560/572/2081/3
D: 5′-CTGATGGAACAGAGATCTTG-3′
dikkopf NewU: 5′-CACCAAGCACAGGAGGAA-3′9556/572/1082/3
D: 5′-TCAGGGAAGACCAGAGCA-3′
Fast3 NewU: 5′-AACCCCCAGAGCTGAAGAAC-3′9556/572/1488/3
D: 5′-TCATATTGGCCCCTCATAGC-3′
Fgf8 Kofron et al., 1999 U: 5′-CTGGTGACCGACCAACTAAG-3′9555/572/1486/3
D: 5′-ACCAGCCTTCGTACTTGACA-3′
Foxh1 NewU: 5′-TTCTACAATCTCCCCCGTTG-3′9556/572/1281/3
D: 5′-AGAAGTGGGCAAGTCCAAT-3′
Goosecoid v.2 NewU: 5′-TTCACCGATGAACAACTGGA-3′9555/572/1182/3
D: 5′-TTCCACTTTTGGGCATTTTC-3′
GS17 XMMR*U: 5′-ATGCCAGTCCAACTTCAAGGCA-3′9560/572/2081/3
D: 5′-CCTCTAGCATAGATGGACTGTA-3′
Mix.2 NewU: 5′-TGATGGACTATGAATGGAGTGAA-3′9555/572/1081/3
D: 5′-CCCCAAACTGTGGTGTTACC-3′
Mixer Xanthos et al., 2001 U: 5′-CACCAGCCCAGCACTTAACC-3′9555/572/1283/3
D: 5′-CAATGTCACATCAACTGAAG-3′
Odc Heasman et al., 2000U: 5′-GCCATTGTGAAGACTCTCTCCATTC-3′9555/572/1283/3
D: 5′-TTCGGGTGATTCCTTGCCAC-3′
Siamois Heasman et al., 2000U: 5′-CTGTCCTACAAGAGACTCTG-3′9555/572/1681/3
D: 5′-TGTTGACTGCAGACTGTTGA-3′
Xbra Sun et al., 1999U: 5′-TTCTGAAGGTGAGCATGTCG-3′9555/572/875/3
D: 5′-GTTTGACTTTGCTAAAAGAGACAGG-3′
Xlim1 Kofron et al., 2004U: 5′-CCCTGGCAGCAACTATGACT-3′9555/572/1185/3
D: 5′-GGTTGCCATAACCTCCATTG-3′
Xnrl v.2 NewU: 5′-AGAGGAATGTGGGTGCAGTT-3′9555/572/1076/3
D: 5′-CAACAAAGCCAAGGCATAAC-3′
Xnr2 v.2 NewU: 5′-TTACTGTATGAAGACGGAGAAGTTG-3′9555/572/1077/3
D: 5′-ATGCGACATGCCACAAAAC-3′
Xnr3 v.2 NewU: 5′-TAATCTGTTGTGCCGATCCA-3′9556/572/979/3
D: 5′-ATCAATGTTGCCCTTTTTCA-3′
Xnr5 v.2 NewU: 5′-TGGTTGGGGAGACCTGATTA-3′9555/572/1077/3
D: 5′-AGAGGCCTCATCTTCACTGG-3′
Xnr6 v.2 NewU: 5′-AAGATTGGATGGGGTCATCA-3′9555/572/1080/3
D: 5′-ATCAGCATGGACAAGGGACT-3′
Xvent2 NewU: 5′-TGAGACTTGGGCACTGTCTG-3′9562/572/2083/3
D: 5′-CCTCTGTTGAATGGCTTGCT-3′
Xsox17α Xanthos et al., 2001 U: 5′-GCAAGATGCTTGGCAAGTCG-3′9558/572/885/3
D: 5′-GCTGAAGTTCTCTAGACACA-3′