Table 1.

Integration of donor cells in operated embryos

Cell type implantedNumber of embryos operatedNumber of embryos surviving operationNumber of embryos with GFP+ cellsPercentage of embryos containing GFP+ cells in VSC with axonsPercentage of embryos containing GFP+ cells in DRG
RA-treated cells1131016365.5±27.3 (n=24)59.2±34.5 (n=27)
RA-untreated cells86715674.9±27.6 (n=16)86.1±16.4 (n=19)
  • Analyses were performed on embryos from 16 independent experiments (nine with RA-treated EBs and seven with RA-untreated EBs). Operated embryos were initially observed as wholemounts and those showing a GFP signal at the implant region (third column) were sectioned. All these embryos contained numerous GFP+ cells in several continuous sections, but the number of these varied. GFP+ cells were frequently detected in the ventral spinal cord (VSC) extending axons towards the periphery (fourth column), and in the DRG (fifth column). In few sections, donor cells were detected only in the DRG, suggesting that they also migrated along the anteroposterior axis. We failed to detect mouse nuclei in embryos that did not exhibit a GFP signal (∼40%), suggesting that the donor cells did not survive in these animals. Results are ±s.e.m.; n represents the number of embryos analyzed.