Table 2.

Dorsalization phenotype of tsg1 knockdown and RNA rescue

Class 4*Class 3Wild type
MO1 only (n=1110)20%47%33%
MO4§ only (n=414)47%23%30%
MO5 only (n=1150)38%48%13%
MO5+tsg1 RNA** (n=243)11%29%60%
MO5+bmp2b RNA†† (n=68)10%11%76%
MO5+smad5 RNA‡‡ (n=57)2%3%86%
  • * Similar to embryos in Fig. 2O,Q

  • Similar to embryos in Fig. 2N,P

  • 32 ng MO1 per embryo

  • § 32 ng MO4 per embryo

  • 25 ng MO5 per embryo

  • ** 2pg tsg1 RNA per embryo

  • †† 12 pg bmp2b RNA per embryo

  • ‡‡ 20 pg smad5 RNA per embryo