Table 1.

Effect of TCE stem-loop II and III mutations on translational repression in vitro

3′UTRTranslationP
3× TCE1.0
3× SRE-3.7 (±1.1)0.02
3× TCEIIA4.2 (±0.8)0.01
3× TCEIIIA1.4 (±0.04)0.0007
tub 3′UTR3.9 (±1.3)0.03
  • Effect of TCE stem-loop II and III mutations on translational repression in vitro. Capped and polyadenylated luciferase reporter RNAs bearing the control tub 3′UTR or three copies of a wild-type or mutant TCE were translated in embryonic extract. For each RNA, luciferase activity was normalized to the value obtained following translation in rabbit reticulocyte extract, which does not exhibit TCE-dependent repression. The corrected values were then expressed as a ratio to the value obtained for 3× TCE, to determine the relative ability of each 3′UTR to confer translational repression. The mean and standard deviation from four independent experiments are shown. P values were determined using the Student's t-test.