Table 2.

Summary of data for female and male genes

RAID***ESTCGBlot§Gene nameGO function**FvM wt††FvM tud††Model‡‡Model P§§FvdsxD¶¶MvFru¶¶
Female genes
75H385.32107.16DSX**
79G7CG17012YesPeptidase17.9118.49DSX**
64B1017.9132.10DSX**
61G12No6.6713.97DSX**
75H7No4.4311.49DSX**
86D9LD27313CG7777YesTransporter3.643.15DSX**
75G4GH23040CG1090Transporter3.605.76DSX**
88A8sopRibosomal_protein3.212.21DSX*
88A7CG5821GRNA_binding2.912.25DSX*
60F4GH08387CG8327Enzyme2.553.51DSX*
85C11LD26157CG10281GTfIIFalphaTranscription_factor2.452.01DSX*
98D8CG14792YesstaRibosomal_protein2.431.81FRU**
45G122.411.40DSX*
109G12CG1573PgkProtein_kinase2.352.09DSX*
85E11LD26426CG1646DNA_binding2.311.67DSX*
98H3CG10944RpS6Ribosomal_protein2.221.87FRU*
89B6CG12269Unknown2.221.77FRU*
107B5LD47943CG7899GEnzyme2.141.91DSX
74E32.102.25FRU
107A9LD47858CG8370GUnknown2.062.10DSX
72B11CG3195GRpL12Ribosomal_protein2.062.03DSX
109F12LP06017CG9699Cytoskeletal_structural_protein2.061.75FRU*
40E112.062.10DSX
49G4LD35056CG9696GDNA_binding2.041.56DSX*
102H9LD44094CG11248Motor_protein2.041.97DSX
78D9CG3751Ribosomal_protein2.031.96FRU
102H11LD44138CG7129Signal_transduction1.951.54DSX
75H6GH23156CG10566NoDefense/immunity_protein1.894.93DSX**
65D7GH13422CG4087RpP2Ribosomal_protein1.852.16DSX
83B2GM13756CG12740YesRibosomal_protein1.801.82DSX
78D7GH25190CG4392YesTransporter1.791.66FRU
41E4GH14439CG18525Unknown1.792.83DSX*
41H5GH14851CG8453Cyp6g1Cytochrome_P4501.671.50DSX
68B61.661.91DSX
34G1GH06693CG9547Acyl-CoA_dehydrogenase1.581.86DSX
96E9LD37170CG8705Cytoskeletal_structural_protein1.381.44DSX
Male genes
38G8GH1288CG7157YesAcp36DESignal_transduction218.3788.83DSX***
65F5GH13755CG17022YesUnknown143.9576.82DSX**
38A9GH10507CG18284YesUnknown62.7068.39DSX**
75E2GH22889CG17843YesElectron_transfer36.5515.64DSX**
54C10GH01346CG10096Unknown15.514.25DSX*
70E7CG8708YesUnknown11.7216.46DSX**
68B5GH15905CG12558YesEndopeptidase11.6211.39DSX*
77H12CG7178Yes9.786.66FRU*
76E9CG9519YesEnzyme9.407.38DSX**
68B9GH15921CG16820Unknown8.224.39DSX*
96C7Yes6.7212.60DSX**
54F6No6.665.00DSX*
30E3YesEndopeptidase6.132.04FRU*
42C7GH152956.109.91DSX**
65E3GH13557CG7342Transporter4.924.04DSX**
77F5GH24480CG12268Enzyme4.642.17DSX*
32E4GH04125CG9456Serpin4.302.15DSX*
77H9GH24781CG6518inaCProtein_kinase4.231.30DSX*
66D12GH14506CG8909YesReceptor3.162.17DSX**
75B4GH22686CG6716YespairedTranscription_factor3.072.31DSX*
74C9GH21941CG5449Receptor2.832.14DSX*
34H9CG3359YesmfasSignal_transduction2.781.79FRU*
54A10Signal_transduction2.331.45DSX*
72H52.241.31FRU*
72B3GH19969CG8110YesSignal_transduction2.122.10DSX
102D1LD43488CG5740GMotor_protein2.021.40FRU
39D8GH11985CG14024Unknown2.011.58DSX*
73F7GH21295CG5411Enzyme1.971.31FRU*
55F5GH03013CG11605Actin_binding1.951.37DSX*
70E1GH18278CG15097Actin_binding1.891.42DSX*
61G11GH09582CG6844nAcRa-96AbIon_channel1.781.49DSX
67D4GH15286CG5455Transcription_factor1.661.36DSX*
81F10GH27701CG8552GLigand_binding_or_carrier1.591.41FRU
40F6GH13452CG7748OstStt3Enzyme1.532.48DSX*
• *** An arbitrary unique identifier (Arbeitman et al., 2002)

• The BDGP unique identifier, if known. All BDGP ESTs were re-sequenced and those that did not match the expected EST have no designation. A —' indicates that the EST is chimeric or from a contaminated well. 75H3 is a CG6666/CG3244 chimera; 64B10 is agbb/Yp1 chimera; 61G12 has two sequence populations in well; 75H7 has no apparent open reading frame; 88A8 is chimeric with non-Drosophila DNA; 45G12 is a ribosomal/P-element chimera; 74E3 is a ribosomal/no significant BLAST chimera; 44E11 is aCG7283 (ribosomal)/CG8536 (B-N-acetylglucosaminyl-glycopeptide β-1-4 galactosytransferase); 68B6 is a ribosomal/ion channel chimera; 96C7 has two sequence populations in well; 30E3 is a CG5023/CG4651 chimera; 54A10 and 72H5 contain a transposon sequence; 98D8 contains sequence from ribosomal and CG9243; 40A5 is an EST from either RH4 or sina, which are coded for on opposite strands; 74E3 contains sequence from ribosomal and CG6684; 40E11 chimera of two ribosomal genes

• The celera-predicted gene based on the EST sequencing results presented here

• § Results of RNA blot analysis. Yes', No' and G' indicate that the RNA blot confirms or does not confirm the somatic sex differential expression, or is due to germline expression, respectively

• The names of genes for those genes that have been previously studied

• ** Predicted protein functions of the products of the ESTs

• †† FvMwt and FvMtud, the fold difference in expression between all of the adult wild-type females and males, and all of the adult tudor female and male microarray data, respectively

• ‡‡ The branch of the hierarchy that is predicted to regulate the gene, based on the statistical model presented (see Materials and methods)

• §§ An asterisk indicates that the P value is less that 0.05 based on the model

• ¶¶ An asterisk indicates that in one-tailed t-tests the P value is less than 0.05. The data in the tables are sorted: those showing the largest fold difference in expression between adult wild-type females and males are at the top, and the rest of the data is in descending order based on that criterion