Table 1.

Summary of microarray analysis and real-time RT-PCR verification

Criteria Genes detected
Fold changen/9*LimbsGenitalsTotalNumber tested by RT-PCRNumber of confirmed 1° samplesNumber of confirmed 2° samples
>2.0-fold≥713443 (75%)3 (75%)
>1.4<2.0-fold≥77613108 (80%)7 (70%)
>1.4-fold612162884 (50%)3 (37%)
>1.4-fold<6>100>100>20031 (33%)1 (33%)
Total251614
  • 1° samples, cDNA made using the same RNA that was used to produce cRNA for microarray hybridization.

    2° samples, independently extracted, triplicate RNA samples used to make cDNA.

    RT-PCR, quantitative real-time reverse-transcription PCR.

  • * Number of nine pairwise comparisons showing a significant increase or decrease (P<0.0025) by Wilcoxon's Signed Rank Test.